This in vitro study was conducted to evaluate lymphocyte blastogenic and cytokine production by bovine peripheral blood mononuclear cells (PBMCs) stimulated with phytohemagglutinin (PHA), pokeweed mitogen (PWM) and concanavalin A (Con A) mitogens, by using tetrazolium salt and ELISA tests, respectively. The results presented that  Interleukin-2 (IL-2), IL-4, IL-5, IL-10, IL-17 and IFN-...

Deposition of behenic acid (B.A) mono and bilayers onto gold coated surfaces was performed by Langmuir-Blodget dip-casting technique. Surface Plasmon resonance (SPR) and ellipsometry methods were employed for investigation of the monolayer and bilayer films. The adsorption of the biologically important molecule Concanavalin A (Con A) from bulk solution to these monolayers and bilayers as we...

This study was designed to examine the effects of retinoic acid adding to cumulus and/or fibroblast cellsmonolayer on the development of mouse early embryos. One-cell mouse embryos were obtained from NMRImice after superovulation by an intraperitoneal injection of 5 IU equine chorionic gonadotrophin (eCG)followed 48 hrs later by 5 IU human chorionic gonadotrophin (hCG). Mouse embryonic fibrobla...

this in vitro study was conducted to evaluate lymphocyte blastogenic and cytokine production by bovine peripheral blood mononuclear cells (pbmcs) stimulated with phytohemagglutinin (pha), pokeweed mitogen (pwm) and concanavalin a (con a) mitogens, by using tetrazolium salt and elisa tests, respectively. the results presented that  interleukin-2 (il-2), il-4, il-5, il-10, il-17 and ifn-γ product...

In this study, it was surveyed to know whether an oral single dose of oleuropein could mimic the cardiac preconditioning in rats’ hearts or whether its prolonged oral administration could protect the heart against the aconitine-induced arrhythmia in rats. Eighty male Wistar rats were divided into two series (n = 8 in each group). In the first series, all groups (except the control (Con) group) ...

The objective of the study was to compare the effects of antibiotic virginiamycin, probiotic Protexin® and Plantago major L. (plantain) on performance, serum metabolites, immune response, and the ileal microbial population of broilers. The experiment was carried out with a total of 200 day-old male Ross 308 broiler chickens in a completely randomized design. Chickens were allocated to five grou...

The capping of Concanavalin A (Con A) receptors induced by Con A was studied using human peripheral blood lymphocytes. The effects of Con A dose (5--100 micrograms/ml), pretreatment at 4 degrees C, unbound Con A, extracellular Ca2+ and Mg2+ and vinblastine were evaluated using Con A-horseradish peroxidase and electron microscopy. Lymphocytes incubated with Con A at 4 degrees C and fixed with gl...