Partially fed (4 -5 days) H .a. anatolicum (Iranian isolate) female adult ticks were used for preparation of antigens of midgut which are, the gut supernate antigen (GS), gut membrane antigen (GM), triton extracted gut membrane antigen ( TX-GM ) and sodium dodecyl sulphate treated Triton extracted gut membrane antigen (SDS-TX-GM). The New Zealand white rabbits were immunized with GS, GM, TX- ...

Schistosoma japonicum-infected mice develop hepatic granulomas, immediate hypersensitivity (IH), and delayed hypersensitivity (DH) to soluble egg antigens (SEA) released by parasite eggs trapped in liver sinusoids. All of these responses spontaneously regress after 7 to 9 weeks of infection. This study aimed to develop an in vivo system for the further dissection of cellular and humoral immune ...

Chemokines and adhesion molecules up-regulated in lymphatic endothelial cells (LECs) during tissue inflammation are thought to enhance dendritic cell (DC) migration to draining lymph nodes, but the in vivo control of this process is not well understood. We performed a transcriptional profiling analysis of LECs isolated from murine skin and found that inflammation induced by a contact hypersensi...

Immunization of rabbits and rhesus monkeys with Streptococcus mutans whole cells, cell walls, and defined streptococcal antigens (SAs) SA I/II, SA I, and SA II resulted in high antibody titers to SA I/II (10(-4) to 10(-6)) when tested by the solid-phase radioimmunoassay. Cross-reactive antibodies to heart homogenates (HH) were not elicited in rhesus monkeys. A few rabbits immunized with cell wa...

Eysenhardtia polystachya is widely used in folk medicine as an anti-rheumatic and analgesicagent, but no systematic study of its effects on several markers associated with rheumatoidarthritis and its ethnomedical use as analgesic agent has been performed. We evaluated theanti-arthritic and antinociceptive properties of an ethanolic extract of E. polystachya (EE) barkand its rich-flavonoids frac...

In order to replace the use of mammals for the production of hyperimmune sera and to replace Freund"s complete adjuvant (FCA) with its undesirable side effects, the production and immunological features of IgY were investigated systematically. An important advantage of IgY is that large amounts can easily be extracted from the yolk of immunized laying hens. With special regard to animal welfare...

A chemically synthesized copy (S-CB7) of a fragment (35 amino acid residues) of type 24 streptococcal M protein was covalently linked to polylysine with carbodiimide and injected subcutaneously into rabbits without adjuvant. Although the primary immune responses as measured by enzyme-linked immunosorbent assays at biweekly intervals were weak, the secondary responses as measured by both enzyme-...

Eysenhardtia polystachya is widely used in folk medicine as an anti-rheumatic and analgesicagent, but no systematic study of its effects on several markers associated with rheumatoidarthritis and its ethnomedical use as analgesic agent has been performed. We evaluated theanti-arthritic and antinociceptive properties of an ethanolic extract of E. polystachya (EE) barkand its rich-flavonoids frac...

Crotalaria juncea (Leguminoceae) has been used for its antiarthritic effect in the folklore medicine. The purpose of this study was investigating experimentally the possible antiarthritic and antiulcerogenic properties of Crotalaria juncea leaves in animal model. Ethanolic extract of Crotalaria juncea (CJE) was administered at the doses of 200 and 400 mg/kg to complete Freund’s adjuvant (...

Anti-inflammatory potency of 5-hydroxypyrimidine derivatives SNK-411 (2-isobutyl-4,6-dimethyl-5-hydroxypirimidine) and SNK-578 (hydrochloride 2-isobutyl-4,6-dimethyl-5-hydroxypirimidine) was evaluated on the model complete Freund`s adjuvant (CFA)-induced exudative inflammation in experiment 60 outbreed male rats weighing 180–200 g comparison with prednisolone. Prednisolone, were injected twice:...