A general-purpose reagent capable of reacting with immunoglobulin G in a modified enzyme-linked immunosorbent assay technique was prepared by using protein A coupled with horseradish peroxidase. The reagent detected low levels (0.003 to 1.0 microgram/ml) of human immunoglobulin G and was also applied in an enzyme-linked immunosorbent assay for titration of antibody to human cytomegalovirus. The...

The counterimmunoelectrophoresis (CIE) technique was developed as a diagnostic procedure for ram epididymitis caused by Brucella ovis. CIE test results with sera from naturally and experimentally infected male sheep compared favorably with those obtained by gel diffusion and complement fixation employing the same B. ovis surface R antigen. The main advantage of CIE over gel diffusion consists i...

The agar-gel precipitin-inhibition technique has been modified to detect antibodies of Histoplasma capsulatum in sera from human clinical cases and experimental animals infected with this organism. By use of this modified technique, the histoplasmosis can be detected more consistently and reliably than with the direct agar-gel diffusion test, and titers are comparable to those attained by the c...

Serum hepatitis (Australia) antigen in the sera of hepatitis patients and carriers can be detected in one and a half to three hours by crossover electrophoresis. The method is more sensitive than the immunodiffusion technique commonly employed in this field. It is of the same order of sensitivity as complement fixation but is less complicated.Crossover electrophoresis is thus the method of choi...

Cytomegalovirus antibodies were measured in human sera by a nuclear anticomplement immunofluorescence test that used as antigen the isolated nucleic of virus-infected fibroblasts cells lysed in distilled water. The method exhibited less nonspecific fluorescence than either a conventional whole-cell anticomplement immunofluorescence test or an indirect fluorescent antibody test applied to the sa...

A Case of Cryptococcal meningitis was diagnosed in a 22 year old Pigeon -fancier from Mazandaran prm ince of Iran (close to the Caspian sea). The patient was treated with systemic 5-nuoc to,;in and amphotericin B together with intrathecal injection of ampho. B. Further investigations showed an abnormal reduction in the number ofT-lymphocytes, while humoral immunity was maintained in the normal ...