background: it is established that sperm dna integrity is essential in fertilization and normal embryo and fetal development. routine semen analysis gives an approximate evaluation of the functional competence of spermatozoa, but does not always reflect the quality of sperm dna. therefore, the evaluation of sperm dna integrity, in addition to routine sperm parameters, could add further informat...

malathion (s-(1, 2-dicarboethoxyethyl) o, o-dimethyl phosphorodithioate) is one of the organophosphate pesticides comprehensively used in agriculture fields throughout the world. in spite of widely used, little information are available about dna damage in aquatic organisms. therefore, the present study carried out to investigation of dna damage induced by malathion in cyprinus carpio (pisces: ...

crude oil is enriched in polycyclic aromatic hydrocarbons (pahs). many pah analogs have proved to potentially damage dna. dna damage can be assessed using various biomarkers to find out the degree of genotoxicity of pollutants following in vitro exposure. in this research the comet assay and micronucleus (mn) test were used to detect dna damages and cytogenetic changes following crude oil expos...

background: quantification of radiation-induced apoptosis in peripheral blood lymphocytes (pbls) has been proposed as a possible screening test for cancer-prone individuals and also for the prediction of normal tissue responses after radiotherapy. materials and methods: the neutral version of the comet assay (single-cell gel electrophoresis) was used 24, 48, 72 hours after irradiation with 8 gy...

background: boron and gadolinium are among the nuclides that hold a unique property of being a neutron capture therapy agent. neutron beams have often a considerable portion of gamma rays with fast neutrons. gamma rays, as beam contaminants, can cause considerable damage to normal tissues even if such tissues do contain high boron concentrations. materials and methods: the modification of radio...

The comet assay is a simple and cost effective technique, commonly used to analyze and quantify DNA damage in individual cells. The versatility of the comet assay allows introduction of various modifications to the basic technique. The difference in the methylation sensitivity of the isoschizomeric restriction enzymes HpaII and MspI are used to demonstrate the ability of the comet assay to meas...

normal 0 false false false en-us x-none ar-sa background: more than 100,000 iranian veterans and civilians still suffer from various long-term complications due to their exposure to sulfur mustard (sm) during the iran–iraq war in 1983–88. the aim of the study was to investigate dna damage of sm in veterans who were exposed to sm, 23–27 years prior to this study. materials and methods: blood sam...

background: cadmium (cd) is a heavy metal which is widely spread in our surroundings and has a very strong ability to accumulate in the body organs such as spleen. in this study we aimed to determine the genotoxicity and cytotoxicity effects on mice spleen  treated with cadmium. methods: thirty male mice were enrolled in this study and kept in standard conditions. mice were randomly divided int...

background: agriculture is an important sector for the malaysian economy. the use of pesticides in agriculture is crucial due to its function in keeping the crops from harmful insects. children living near agricultural fields are at risk of pesticide poisoning. objective: to evaluate the genotoxic risk among children who exposed to pesticides and measure dna damage due to pesticides exposure. m...

The comet assay has been widely used to measure a range of cellular responses to DNA damage and has found applications in genotoxicity studies, bio-monitoring, ecological testing and in the study of human disease. This review discusses how the comet assay has been applied to the study of DNA damage and repair associated with cancer. The potential of the assay as a tool for predicting an individ...