نتایج جستجو برای: colorimetric aptasensor
تعداد نتایج: 10653 فیلتر نتایج به سال:
In this communication, we develop a novel fluorescent aptasensor for thrombin detection with the use of poly(m-phenylenediamine) (PMPD) rods as an effective sensing platform. This aptasensor exhibits extraordinarily high sensitivity with a detection limit as low as 100 pM and excellent selectivity.
Aptamers are ligand-binding oligonucleotide sequences with high affinities and selectivities towards their targets. Concomitantly, excellent recognition property has led to being adapted in analytical applications, drug, diagnostics, many other platforms the area of forensic science, respects, they superior analogous antibody-based assays. Recently, major progress been made biosensing applicati...
A bifunctional and simple aptasensor was designed to one-spot simultaneously detect two analytes, lysozyme and ATP. The aptasensor was obtained by the electronic interaction between methyl violet (MV) and dsDNA. The dsDNA was obtained by hybridization of ATP aptamer and lysozyme aptamer. And we used the resonance light scattering (RLS) technique to detect the concentration of lysozyme and ATP. ...
A simple and highly sensitive homogeneous aptasensor is developed, which relies on nicking enzyme. The sensitivity of this newly proposed aptasensor is about three orders of magnitude higher than that of traditional homogeneous aptasensors. Furthermore, it is capable of detecting target protein in real samples.
This research develops a label-free aptamer biosensor (aptasensor) based on graphite-epoxy composite electrodes (GECs) for the detection of lysozyme protein using Electrochemical Impedance Spectroscopy (EIS) technique. The chosen immobilization technique was based on covalent bonding using carbodiimide chemistry; for this purpose, carboxylic moieties were first generated on the graphite by elec...
A novel strategy for the quantitative determination of human colon cancer DLD-1 cells utilizing an electrochemical aptasensor was developed by effective surface recognition between Mucin 1 glycoprotein over-expressed on the cell membrane and MUC-1 aptamer (MUC-1) bound on carbon nanospheres (CNSs). An MTT assay revealed that the as-prepared CNSs by green route exhibited satisfactory biocompatib...
Oncoprotein platelet derived growth factor-BB (PDGF-BB) is one of the most critical growth factors that regulates tumor growth and division. In this work, a highly sensitive and selective fluorescence resonance energy transfer (FRET) aptasensor for PDGF-BB detection based on the assembly of dye-labeled aptamer and graphene oxide (GO) is developed for the first time. Due to the non-covalent asse...
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