The use of a minimal lactose enrichment broth (MLB) in conjunction with immunomagnetic electrochemiluminescence detection (IM-ECL) was evaluated for the estimation of viable Escherichia coli O157 populations in surface water samples. In principle, E. coli O157 populations (C(initial E. coli O157)) can be derived from enrichment data according to the equation: C(initial E. coli O157) = C(initial...

The distribution of coliforms in the sediments around three marine sewage outfalls was investigated. It was found that coliform fields extended for considerable distances around the two outfalls discharging unchlorinatcd primary treatment effluent and that a less extensive field existed around the outfall discharging more highly treated sewage. In general, the coliforms in the sediments were fo...

OBJECTIVE To verify the existence of a relationship between presence of off-flavor and microorganism load in quality control rejected samples of expressed human milk from a donor milk bank. METHODS A total of 30 samples of expressed human milk with off-flavor were tested for the occurrence of the following microorganisms: aerobic mesophilic, psycrotrophic, proteolytic, psycrotrophic proteolyt...

Twenty-four freshwater sampling sites (11 river water, 6 spring water, and 7 groundwater) were selected from 4 sampling areas located in the northern and central parts of Taiwan. A total of 125 water samples were collected during a 5-month sampling period, and the numbers of total heterotrophic bacteria, total coliforms, fecal coliforms, enterococci, Aeromonas hydrophila, and Salmonella spp. we...

This study aims to determine the concentrations of total coliforms and Escherichia coli (E. coli) in beach water, Teluk Kemang beach. This study was also aimed to determine relationship between total coliforms, E. coli and physicochemical parameters. As perceived health symptoms among beach visitors are rarely incorporated in beach water studies, this element was also assessed in this study. A ...

STUDY OBJECTIVE To find out whether bathing in sewage polluted waters implies a danger to bathers' health and to determine the best microbiological indicator to predict the relation between bathing and the appearance of some symptoms. DESIGN Cohort study. SETTING City of Santander (north of Spain). PARTICIPANTS From the people going to four Santander beaches in the period from 1 July to 1...

Coliform group has been extensively used as an indicator of drinking water quality and historically led to the public health protection concept. Multiple tube fermentation technique has been currently used for assessment of the microbial quality of drinking water. This method, however, has limitations. Enzymatic assay constitute an alternative approach for detecting indicator bacteria, namely t...

For the past century, public health officials have relied on fecal coliforms to identify and track bacterial contamination. However, several issues arise when using coliforms as indicators for viral pathogen risk. First, there is a lack of association between fecal coliforms and human enteric viruses in the environment. Second, there are differences in the fate of coliforms compared to viral pa...

D. H. M. JOYNSON AND C. H. L. HOWELLS (Public Health Laboratory, University Hospital of Wales, Cardiff) Samples of animal feeding stuffs were examined for the presence of Gram-negative, lactosefermenting organisms (hereafter called coliforms) and the antibiotic resistance of any strain isolated was determined. Coliforms were found in 13% of all the feeds examined and of these organisms 64-4% we...

Most of present traditional ice cream from hygienic quality has non-conforming with extant standards. Pasteurization of primary ice cream mixture with sufficient thermal processing increase hygienic quality level and causes control of primary infection. Origins of infection for traditional ice cream consist of: use of infected primary materials (milk or cream) or non-pasteurization of them. Stu...