BACKGROUND Helicobacter pylori, causing the most common chronic bacterial infection, exist in two forms; bacilli and coccoid. The coccoid form is identified as viable but non-culturable bacteria. OBJECTIVES The current study aimed to conduct culture, polymerase chain reaction (PCR), and loop-mediated isothermal amplification (LAMP) tests to identify coccoid forms of H. pylori. MATERIALS AND...

A brief note was published some years ago by W. T. Morgan of the Lister Institute, London, and the writer (1932) describing the isolation from decayed woody tissues of a micro-organism which decomposes the specific polysaccharides of Shigella dysenteriae, Shiga; Shigella dysenteriae, Flexner Y; Pneumococcus Type II; and the tubercle bacillus. At the time a study of the relationship of this orga...

The viability of the coccoid forms of Helicobacter pylori was evaluated by assessing protein synthesis. Metabolic labeling studies showed the synthesis of proteins and the specific protein profiles of H. pylori coccoids produced under various conditions. Harsh conditions such as aerobiosis and starvation (lack of horse serum) in the culture did not affect the synthesis of proteins in the coccoi...

Intravenous and intraarterial catheters were examined microbiologically and morphologically. Bacteria or yeasts were recovered from 38 of the 63 catheters examined, and Staphylococcus epidermidis was present on 29 of the 38 colonized catheters. Examination of unused Teflon catheters ( Jelco ; Surgikos , Inc., Peterborough , Ontario, Canada) showed surface irregularities, and the examination of ...

Chromera veliais a photosynthetic alga with a secondary plastid that represents the closest known photosynthetic relative of the apicomplexan parasites. The original description of this organism was based on brownish, immotile coccoid cells, which is the predominating stage ofC. veliain the culture. Here we provide a detailed light and electron microscopy description of coccoid cells ofC. velia...

3 Escola Superior de Tecnologia e Gestão, Instituto Politécnico de Viana do Castelo, Portugal 8 9 E-mails: [email protected]; [email protected]; [email protected]; 10 [email protected]; [email protected] and [email protected]. 11 12 Abstract After characterization of preferred conditions for Helicobacter pylori survival in the 13 sessile state, it was observed t...

background: helicobacter pylori ( pylori ) was the most common cause of chronic gastritis and was linked to peptic ulcer disease and gastric cancer. environmental factors such as water a reservoir of h.pylori which infect human.  a non-culture bacteria in coccoid forms widespread in aquatic environments.the objective of this study  was elevating the diagnostic value of pcr and culture methods f...

conclusions results of the current study confirmed that molecular methods such as pcr and lamp were much more sensitive, rapid, and specific than culturing to identify non-culturable coccoid forms of h. pylori in water. results ten copies of targeted dna were required for pcr detection whereas only five copies gave a positive reaction by lamp assay, with 100% specificity. of the 10 isolates ino...

BACKGROUND Helicobacter pylori (H. pylori) is a spiral Gram negative bacteria that can transform to the coccoid form in adverse conditions. OBJECTIVES The aim of this study was to determine the in vitro morphological and bactericidal effects of metronidazole, amoxicillin and clarithromycin on H. pylori. MATERIALS AND METHODS The standard strain 26695 of H. pylori was cultured on Brucella ag...

Helicobacter pylori is recognized as the most common pathogen to cause gastritis, peptic and duodenal ulcers, and gastric cancer. The organisms are found in two forms: (1) spiral-shaped bacillus and (2) coccoid. H. pylori coccoid form, generally found in the environment, is the transformed form of the normal spiral-shaped bacillus after exposed to water or adverse environmental conditions such ...