Clathrin-coated vesicles carry traffic from the plasma membrane to endosomes. We report here the real-time visualization of cargo sorting and endocytosis by clathrin-coated pits in living cells. We have detected the formation of coats by monitoring incorporation of fluorescently tagged clathrin or its adaptor AP-2; we have also followed clathrin-mediated uptake of transferrin and of single LDL ...

We have previously demonstrated that the preendosomal compartment in addition to clathrin-coated vesicles, comprises distinct nonclathrin coated endocytic vesicles mediating clathrin-independent endocytosis (Hansen, S. H., K. Sandvig, and B. van Deurs. 1991. J. Cell Biol. 113:731-741). Using K+ depletion in HEp-2 cells to block clathrin-dependent but not clathrin-independent endocytosis, we hav...

Choptuik has demonstrated that naked singularities can arise in gravitational collapse from smooth, asymptotically flat initial data, and that such data have codimension one in spherical symmetry. Here we show, for perfect fluid matter with equation of state p = ρ/3, by perturbing around spherical symmetry, that such data have in fact codimension one in the full phase space, at least in a neigh...

.............................................................................................................. 2

I propose to use as a window the dark core of an optical vortex to examine a weak background signal hidden in the glare of a bright coherent source. Applications such as the detection of an astronomical object, forward-scattered radiation, and incoherent light are described whereby signal enhancements of at least 7 orders of magnitude may be achieved.

growing demand of invisible braces byesthetically conscious patients has led to remarkable inventions in materials for esthetic labial archwires. archwires with excellent optical clarity and mechanical properties comparable to conventional archwires have been manufactured by almost all the leading companies of orthodontic products in the past two decades, but their clinical use is still limited...

The Royal Palace of Norway has a large 19th century wooden furniture collection and most it is coated with shellac-based varnishes. Since these objects are part the royal collections, they still in daily use at different residences and, therefore, need to be frequently cleaned. In this work, effect three cleaning commercial products (Baolin, Centurio, Fulgentin) used collections on shellac-coat...

|A precorrected-FFT algorithm is presented for the calculation of electromagnetic scattering from conducting objects coated with lossy materials. The problem is formulated using an EFIE-PMCHW formulation, which employs the electric ­ eld integral equation (EFIE) for conducting objects and the PMCHW formulation for dielectric objects. The integral equations are then discretized by the method of ...

Dimensional measurements on nano-objects by atomic force microscopy (AFM) require samples of safely fixed and well individualized particles with a suitable surface-specific particle number on flat and clean substrates. Several known and proven particle preparation methods, i.e., membrane filtration, drying, rinsing, dip coating as well as electrostatic and thermal precipitation, were performed ...