BACKGROUND Genome-wide gene expression profiling allows for identification of genes involved in the defense response of the host against pathogens. As presented here, transcriptomic analysis and bioinformatics tools were applied in order to identify genes expressed in the mammary gland parenchyma of cows naturally infected with coagulase-positive and coagulase-negative Staphylococci. RESULTS ...

BACKGROUND To investigate the incidence and antibiotic resistance of staphylococcal strains isolated from milk and milk products and to trace the ecological origin of the Staphylococcus aureus isolated. METHODOLOGY Eighty-one samples of raw milk, lben (whey) and jben (cheese) were analyzed for the presence of staphylococcal strains. Isolates were identified by Gram stains, tests for coagulase...

The aim of the study was to characterise the 493 tube-coagulase-negative Staphylococcus sp. strains isolated from the udder quarters of milking dairy cows in one herd with a high bulk milk somatic cell count (SCC). The isolates were examined phenotypically by cultural features, Gram stain, catalase, coagulase, and API biochemical tests, and genotypically by PCR for chosen genes. About 10% of th...

objective(s): staphylococcus aureus is an important bacterial pathogen responsible for a variety numbers of nosocomial and community acquired infections. biofilm formation is regarded as an important factor in the establishment of s. aureus infection. the contribution of the genetic background of s. aureus to biofilm formation is poorly understood. the aim of the present work was to genotype s....

The SaeRS two-component system plays important roles in regulation of key virulence factors and pathogenicity. In this study, however, we found that the deletion mutation of saeRS enhanced bacterial survival in human blood, whereas complementation of the mutant with SaeRS returned survival to wild-type levels. Moreover, these phenomena were observed in different MRSA genetic background isolates...

OBJECTIVES Integrons are thought to play an important role in the spread of antibiotic resistance. This study investigates class 1 and 2 integron-positive methicillin-resistant coagulase-negative staphylococci strains isolated in Iran and characterizes their patterns of antimicrobial resistance. METHODS Hundred clinical isolates of coagulase-negative staphylococci were characterized for integ...

results of total 60 s. aureus isolates, 51 (85%) were mrsa, and of 60 cons, 7 (11.66%) were mrcons. the results showed that the mic of chlorhexidine for all 120 isolates was 1-16 μg/ml. 15 (12.5%) isolates carried qaca/b gene, 26 (21.7%) carried qacc gene, and 38 (31.7%) carried smr gene. conclusions maintenance of mrsa isolates in the attendance of low amounts of antiseptics could result in th...

Objective(s): Staphylococcus aureus is an important bacterial pathogen responsible for a variety numbers of nosocomial and community acquired infections. Biofilm formation is regarded as an important factor in the establishment of S. aureus infection. The contribution of the genetic background of S. aureus to biofilm formation is poorly understood. The aim of the present work was to genotype S....

BACKGROUND Otitis externa is a common inflammatory ear disease in dogs caused by a variety of pathogens, and coagulase-positive staphylococci are frequently isolated from such infections. OBJECTIVE To identify antimicrobial susceptibility profiles and methicillin-resistant strains among coagulase-positive staphylococci isolated from otitis externa in dogs. METHODS A cross-sectional study wa...