The phototrophic bacterium Chloroflexus aurantiacus uses the 3-hydroxypropionate cycle for autotrophic CO(2) fixation. This cycle starts with acetyl-coenzyme A (CoA) and produces glyoxylate. Glyoxylate is an unconventional cell carbon precursor that needs special enzymes for assimilation. Glyoxylate is combined with propionyl-CoA to beta-methylmalyl-CoA, which is converted to citramalate. Cell ...

The mbd cluster encoding genes of the 3-methylbenzoyl-CoA pathway involved in the anaerobic catabolism of 3-methylbenzoate and m-xylene was characterized for the first time in the denitrifying β-Proteobacterium Azoarcus sp. CIB. The mbdA gene product was identified as a 3-methylbenzoate-CoA ligase required for 3-methylbenzoate activation; its substrate spectrum was unique in activating all thre...

Quantitative traits locus for milk production traits has been described on centromeric end of bovine chromosome 14. Reports name the acyl coA: diacylglycerol acyltransferase (DGAT1) gene as a potential candidate gene with dinucleotide substitution (AA to GC) in exon VIII which causes the change of lysine to alanine in amino acid (K232A).The aim of the present study was to estimate the frequency...

Acetoacetyl-CoA is the precursor of 3-hydroxy-3-methylglutaryl (HMG)-CoA in the mevalonate pathway, which is essential for terpenoid backbone biosynthesis. Acetoacetyl-CoA is also the precursor of poly-beta-hydroxybutyrate, a polymer belonging to the polyester class produced by microorganisms. The de novo synthesis of acetoacetyl-CoA is usually catalyzed by acetoacetyl-CoA thiolase via a thioes...

A gene, phl, encoding a phenylacetyl-CoA ligase was cloned from a phage library of Penicillium chrysogenum AS-P-78. The presence of five introns in the phl gene was confirmed by reverse transcriptase-PCR. The phl gene encoded an aryl-CoA ligase closely related to Arabidopsis thaliana 4-coumaroyl-CoA ligase. The Phl protein contained most of the amino acids defining the aryl-CoA (4-coumaroyl-CoA...

The enzyme 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase catalyses the condensation of acetoacetyl-CoA and acetyl-CoA to form HMGCoA plus free CoA. The activity of HMG-CoA synthase is located in two different compartments: the cytosol and the mitochondria. The HMG-CoA produced by cytosolic HMG-CoA synthase is transformed into mevalonate by the action of HMG-CoA reductase, thus starting the ...

Twenty one staphylococcus aureus isolates of bovine mastitic milk origin from herds at different locations were analysed for their coa gene products and RFLP patterns. One of the isolates was coa gene deficient whereas the rest revealed polymorphism in the coa gene. The isolates revealed three different types of coa gene products (600, 680 or 850 bp) and three distinct RFLP patterns were obtain...

Pseudomonas putida is evolutionarily endowed with features relevant for bioproduction, especially under harsh operating conditions. The rich metabolic versatility of this species, however, comes at the price limited formation acetyl-coenzyme A (CoA) from sugar substrates. Since acetyl-CoA a key precursor number added-value products, in work we deployed an silico-guided rewiring program central ...

Codon usage bias (CUB) refers to an unequal occurrence of specific synonymous codons in a genome with variations within and among species. Findings suggest that CUB is significant interpreting evolutionary trends species and/or genes. However, information remained unclear for organisms the family Potamididae. This study examined compositional features codon patterns coI gene potamidids identify...

Coenzyme A (CoA) is a key molecule in cellular metabolism including the tricarboxylic acid cycle, fatty synthesis, amino synthesis and lipid metabolism. Moreover, CoA required for biological processes like protein post-translational modifications (PTMs) acylation. levels affect amount of histone acetylation thereby modulate gene expression. direct influence on other PTMs, CoAlation 4?-phosphopa...