introduction: this study was initiated to examine the effect of the ampullary and the isthmic primary cell cultures of human oviduct during long-term co-culture on two-cell mouse embryos. materials and methods: using a mechanical procedure, epithelial cells from ampullary (a) and isthmic (i) regions of the human oviduct were isolated and cultured in ham's f-10 with 10% fetal calf serum (fsc). t...

Most of the systematic studies used in the development of human embryo culture media have been done first on mouse embryos. The general use of NMRI outbred mice is a model for toxicology, teratology and pharmacology. NMRI mouse embryo exhibit the two-cell block in vitro. The objective of this study was to evaluate and compare the effects of four kinds of culture media on the development of zygo...

objective: the organotypic co-culture is a well-known technique to examine cellular interactions and their roles in stem cell proliferation and differentiation. this study aims to evaluate the effects of dermal fibroblasts (dfs) on epidermal differentiation of adipose-derived stem cells (ascs) using a three-dimensional (3d) organotypic coculture technique. materials and methods: in this experim...

Umbilical cord blood (UCB) has been used for transplantation in the treatment of hematologic disorders as a source of hematopoietic stem cells (HSCs). Because of insufficient number of cord blood CD34+ cells, the expansion of these cells seems to be important for clinical application. Mesenchymal stromal cells (MSCs), playing an important role in HSCs maintenance, were used as feeder layer. Apo...

the present study has objectively investigated and reviewed 164 managers, employees and workers of tuka steel investment holding company (tsih co.) as one of the most successful companies in iran's steel industry. the survey approach has been adopted to serve the following purposes of this study: a) to define the prevailing organizational culture in steel industry; and b) to review alignment do...

background: this study was designed to develop a new co-culture system, assess the effect of luteinizing hormone (lh) using sequential media to promote development and increase the quality of 2-cell murine embryos through the 8-16 cell stage to morula and blastocyst stages. materials and methods: monolayers for co-culture were prepared from ovine oviduct epithelial cells (ooec) in dmem/f12 medi...

background: specific growth factors and feeder layers seem to have important roles in in vitro embryonic stem cells (escs) differentiation. in this study,the effects of bone morphogenetic protein 4 (bmp4) and mouse embryonic fibroblasts (mefs) co-culture system on germ cell differentiation from mouse escs were studied. materials and methods: cell suspension was prepared from one-day-old embryoi...

background: the ultrastructural analysis of cultured follicles could direct us to understand subcellular changes during in vitro culture. objective: this study was done to verify the ultrastructural characteristics of in vitro cultured mouse isolated preantral follicles in co-culture system in the presence and absence of leukemia inhibitory factor (lif) materials and methods: mechanically isola...