Information about nutrient availability is assessed via largely unknown mechanisms to drive developmental decisions, including the choice of Caenorhabditis elegans larvae to enter into the reproductive cycle or the dauer stage. In this study, we show that CMK-1 CaMKI regulates the dauer decision as a function of feeding state. CMK-1 acts cell-autonomously in the ASI, and non cell-autonomously i...

Mutants of Salmonella typhimurium defective in cytidine 5'-monophosphate (CMP) kinase (cmk) have been isolated. The mutants also lack the ability to phosphorylate 2'-deoxyCMP, indicating that one enzyme is responsible for the phosphorylation of both CMP and deoxyCMP to the corresponding diphosphates. In glucose minimal medium the mutants grow at the same rate as the parental strain; however, th...

Nucleoside analogues have proven to be a highly successful class of anti-cancer and anti-viral drugs. The therapeutic efficacy of nucleoside analogues is dependent of their intracellular phosphorylation. Two cellular nucleoside kinases, deoxycytidine kinase (dCK) and UMP-CMP kinase (CMK) are critical for phosphorylation of cytidine analogues. These kinases provide two first steps of activation ...

The cultivation temperature (T(c)) modulates the thermosensory responses exhibited by C. elegans on thermal gradients. The AFD sensory neurons are essential for thermosensory behaviors, but the molecular mechanisms by which temperature is sensed and the memory of the T(c) is encoded are unknown. Here, we show that the CMK-1 Ca2+/calmodulin-dependent protein kinase I (CaMKI) and the TAX-4 cyclic...

The chymotrypsin-like serine protease inhibitor, succinyl-alanine-alanine-proline-phenylalanine chloromethyl ketone (AAPF(CMK)), has been shown to have anticarcinogenic activity in a number of model systems and to be relatively selective for a nuclear protease. This inhibitor also has substantial effects on growth of tumorigenic human papillomavirus (HPV)-infected keratinocytes in organotypic r...

Mineralization, a characteristic phenotypic property of osteoblastic lineage cells, was blocked by 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF) and decanoyl-Arg-Arg-Leu-Leu-chloromethyl ketone (dec-RRLL-cmk), inhibitors of SKI-1 (site 1; subtilisin kexin like-1) protease. Because SKI-1 is required for activation of SREBP and CREB (cAMP-response element-binding protein)/ATF fa...

Interleukin-3 (IL-3) is a potent stimulator of megakaryocyte proliferation, and autocrine production of IL-3 by megakaryocytic leukemia cell lines and bone marrow-derived megakaryocytes has recently been demonstrated. To characterize the transcriptional regulation of IL-3 in megakaryocytes, we transiently transfected IL-3 promoter CAT constructs that contain variable amounts of 5' flanking sequ...

Mesoporous carbons were synthesized via both soft and hard template methods and compared to a commercial powder activated carbon (PAC) for the adsorption ability of bisphenol-A (BPA) from an aqueous solution. The commercial PAC had a BET-surface of 1027 m²/g with fine pores of 3 nm and less. The hard templated carbon (CMK-3) material had an even higher BET-surface of 1420 m²/g with an average p...

Ordered Mesoporous Carbon (OMC) represents an interesting material for electric double layer capacitors which has the high surface area, easily accessed ordered pore channels and lower production cost. In this work, CMK-3 as promising OMC has been fabricated using the ordered mesoporous silica SBA-15 as a template. The structure and morphology of CMK-3 are characterized by X-ray diffraction...