When Citrobacter freundii cephalosporinase was incubated with 6 beta-[3-(2-chlorophenyl)-5-methyl-4-isoxazolyl]penicillin sulfone (cloxacillin sulfone) in phosphate buffer, the enzyme was suddenly inactivated just after the completion of enzymatic degradation of the cloxacillin sulfone. Such delayed inactivation was due to a secondary inhibitor formed from cloxacillin sulfone during the incubat...

The concentrations of cefamandole, cloxacillin and fusicid acid were measured in the serum and heart tissue of 100 recipients of these drugs before cardiac surgery. During cardiopulmonary bypass, mean (+/- standard deviation) peak concentrations in serum of all patients were 63.0 +/- 34.0 micrograms of cefamandole per ml, 30.8 +/- 17.7 micrograms of cloxacillin per ml, and 32.4 +/- 10.8 microgr...

A combined-disk method using imipenem and cloxacillin was evaluated to discriminate between carbapenemase-producing (n = 56) and nonproducing (n = 62) strains of Pseudomonas aeruginosa. With a cloxacillin load of 4,000 μg/disk, this very simple method showed a sensitivity and a specificity of 100%, irrespective of the type of carbapenemase produced.

A prospective, randomized clinical trial among drug abusers was conducted to assess the efficacy and safety of a short course of a combination of a glycopeptide (vancomycin or teicoplanin) and gentamicin compared with a combination of cloxacillin and gentamicin for treatment of right-side endocarditis caused by Staphylococcus aureus. Therapeutic success was significantly more frequent with clox...

BACKGROUND AND THE PURPOSE OF THE STUDY Hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) has been a major problem worldwide in chemotherapy of infection disease. This study was designed to assess the enhancing effects of a new group of dihydropyridine-3,5dicarboxamides, in combination with cloxacillin with distinctly different mechanisms of action against MRSAs. MATERIAL A...

The authors use an environmental friendly, solvent-free method to prepare metal (II) complexes of cloxacillin and determine the activity complexes. are more active against bacteria isolates than free cloxacillin. spectral studies showed (ligand) as monobasic bidentate material that form a complex with ion through carbonyl amide carboxylate groups carboxylic acid, microanalysis results prove lig...

The spectrophotometric and microbiological assay methods used for the determination of ampicillin levels in ampicillin/cloxacillin combinations were compared. A total of 24 samples of ampicillin/cloxacillin suspensions from 8 batches and 3 companies (B2, D and E) were assayed for their ampicillin content levels using the Spectrophotometric assay method. Infra-red (IR) Spectroscopy confirmed the...

The effect of penicillins and cephalosporins on the various transpeptidation reactions presented in the preceding paper is discussed. Inhibition by cloxacillin and penicillin G of [Wldiaminopimelic acid incorporation during peptidoglycan synthesis appeared to be partially reversible after the antibiotic was destroyed by treatment with penicillinase. Inhibition of the cross-linking reaction by c...

OBJECTIVES The aim of this study was to compare the current screening methods and to evaluate confirmation tests for phenotypic plasmidal AmpC (pAmpC) detection. METHODS For this evaluation we used 503 Enterobacteriaceae from 18 Dutch hospitals and 21 isolates previously confirmed to be pAmpC positive. All isolates were divided into three groups: isolates with 1) reduced susceptibility to cef...