Binding of components of the fibrinolytic system to fibrin is important for the regulation of fibrinolysis. In this study, decomposition of the fibrin network and binding of plasminogen and plasminogen activators (PAs) to fibrin during lysis of a plasma clot were investigated with confocal microscopy using fluorescein-labeled preparations of fibrinogen, plasminogen, tissue-type PA (t-PA), and t...

The mechanism by which activated protein C stimulates fibrinolysis was studied in a simple radiolabeled clot lysis assay system containing purified tissue-type plasminogen activator, bovine endothelial plasminogen activator inhibitor (PAI), plasminogen, 125I-fibrinogen and thrombin. Fibrinolysis was greatly enhanced by the addition of purified bovine activated protein C; however, in the absence...

420 Thrombin-activatable fibrinolysis inhibitor (TAFI) is a 60kDa metallocarboxypeptidase produced by the liver and present in plasma. Independently discovered by several groups, the enzyme is also known as plasma procarboxypeptidase B, procarboxypeptidase R, or procarboxypeptidase U (1-5). TAFI as nomenclature was introduced by Bajar et al. (5). TAFI is expressed as a latent precursor requirin...

Currently, the study of fibrinolysis is beset by problems of methodology (1 ). Disadvantages of many of the present methods for measurement of clot lysisincluding lysis time for the dilute blood clot, thromboelastography, euglobulin clot lysis, fibrin plate method, and caseinolytic assay (1 )-are imprecision of results and time required to perform the test (2). The electromechanical lysis timer...

Methods We conducted an observational, retrospective study in two academic trauma centers from a French university institution. All patients diagnosed with HF on rotational thromboelastometry (ROTEM) between 2010 and 2014 were included. Demographic and clinical parameters, standard laboratory and ROTEM results were collected. HF diagnosis was established when the 60 minute clot lysis index (CLI...

BACKGROUND AND PURPOSE The purpose of this study was to develop a novel MRI method for imaging clot lysis in a rat embolic stroke model and to compare tissue plasminogen activator (tPA)-based clot lysis with and without recombinant Annexin-2 (rA2). METHODS In experiment 1 we used in vitro optimization of clot visualization using multiple MRI contrast agents in concentrations ranging from 5 to...

Objective—The relative balance between clot formation and fibrinolysis is considered to reflect thrombotic potential following vascular injury. The aims of the present study were to (1) to determine the contribution of genetic and environmental factors to variance in measures of clot structure/function in the Leeds Family Study, and (2) to determine the relationship between measures of clot str...

OBJECTIVE The relative balance between clot formation and fibrinolysis is considered to reflect thrombotic potential following vascular injury. The aims of the present study were to (1) to determine the contribution of genetic and environmental factors to variance in measures of clot structure/function in the Leeds Family Study, and (2) to determine the relationship between measures of clot str...

BACKGROUND AND PURPOSE Fibrin clot formation is important in acute intracerebral hemorrhage (ICH). We investigated plasma fibrin clot characteristics in acute ICH compared with acute ischemic stroke (IS) and nonstroke conditions. METHODS In the 3 studied groups, we analyzed plasma fibrin clot phenotype and its association with clinical stroke presentation. RESULTS Compared with controls, in...