clostridium perfringens is an important cause of enteric diseases in both human and animals. the bacteria produce several toxins which play key roles in the pathogenesis of diseases and are classified into five toxin types, on the basis of the differential production of alpha, beta, epsilon and iota toxins. in this study a single pcr assay was developed and used for detection of cpb2 gene to id...

conclusions metronidazole and vancomycin still seem to be most effective drugs for treatment cdi. objectives the aim of this study was to investigate the antimicrobial susceptibility patterns of c. difficile clinical isolates against antibiotics commonly used for treatment cdi in hospitalized patients. material and methods during a 12 month study, 75 c. difficile isolates were collected from 39...

botulism is a serious paralytic disease caused by clostridium botulinum toxin in foods. there are seven recognized serotypes of botulinum neurotoxins among which the principal prevalent types in humans include a, b and e. infant botulism results from intestinal colonization and toxin production by c. botulinum spores in babies less than 1 year old. honey is the most important food discriminated...

conclusions the results of pcr-ribotyping indicate that no relationship exists between clinical and meat isolates. we therefore conclude that other sources than meat may function as a vector for ca-cdi. results the overall prevalence of c. difficile with two toxigenic genes including tcda and tcdb was estimated at 4.0%. c. difficile was detected in 2.8%, 2.1%, 3.6% and 6.2% of chopped beef, gro...

BACKGROUND Owing to problems in accurate species identification of the diverse genus clostridium, the epidemiology and pathogenicity of many species are not fully understood. Moreover, previous studies on clostridium bacteraemia have been limited and relied only on phenotypic species identification. AIMS To characterise the epidemiology, disease spectrum, and outcome of clostridium bacteraemi...

clostridium perfringens is a gram-positive, obligate anaerobic bacterium, which is widely distributed in the environment. c. perfringens is subdivided to 5 groups (types a to e), based on its four major toxin (alpha, beta, epsilon and iota). c. perfringens type b beta toxin causes inflammation and bloody necrotic enteritis. type b related enterotoxaemia is a major problem of veterinary sciences...

Clostridium butyricum is a kind of anaerobic bacteria, so de-aeration is extremely important during culture. In this paper, adding sodium thiosulfate and cysteine as well as mixed culture with Saccharomyces cerevisiae were adopted in de-aeration during Clostridium butyricum culture. The experimental results showed that the optimal dosage of sodium thiosulfate was 0.004g/mL, where the biomass of...

alpha toxin derived from clostridium perfringens was used for diagnosis of streptococcus agalactiae (st.agalactiae) by rapid and spot camp tests. the selected cl.perfringens strain was cultured in optimized conditions; the supernatant of culture was concentrated by ultra filter and purified by deae cellulose chromatography. the efficacy of both purified and crude α-toxins were examined by rapid...