Aim: The present study was aimed at polymerase chain reaction (PCR) amplification and cloning of NAD-dependent betahydroxybutyryl coenzyme A dehydrogenase (BHBD) gene of Clostridium chauvoei. Materials and Methods: C. chauvoei was cultured and confirmed by 16-23S rDNA spacer region primers. The primers for nad-bhbd gene of C. chauvoei were designed to aid in cloning into pRham-N-His SUMO-Kan ve...

Gas chromatography of the serum of mice inoculated 6 hr earlier with Staphylococcus aureus, Escherichia coli K-12, and Clostridium chauvoei revealed the formation of products associated with the infections. Longer periods were required before compounds resulting from infecting the animals with Salmonella typhimurium and other Clostridium species were detected by gas chromatographic means. The t...

PURPOSE To investigate the genetic diversity of toxigenic Clostridium strains isolated from soil, water, meat and its associated polluted sites of Southern India. MATERIALS AND METHODS A total of 27 identified isolates of six different toxigenic clostridial species including C. bifermentans , C. botulinum , C. chauvoei , C. ramosum , C. tetani and C. novyi were isolated and characterized by c...

A Longitudinal epidemiologic study was developed for the isolation, biochemical characterization and molecular typing the bacterial pathogen Clostridium spp found in soil from areas affected by bovine sudden mortality. Weincluded 10 herds, located in three localities. The genetic variability of Clostridium genus was analyzed by DNA sequence of a 1500 bp fragment from the 16S rRNA gene. Twenty f...

Evidence for Mitotic Crossing-over During the Parasexual Cycle of the Cellular Slime Mould Dictyostelium discoideum. By E. B. GINGOLD and J. M. ASHWORTH Growth of a Wild Strain and of a Pimelic Acid-utilizing Mutant of Pseudomonas azelaica on Aliphatic Dicarboxylic Acids. By L. JANOTA-BASSALIK and B. BOHDANOWICZ-STRUCI~~SKA . Plasmid-instability in acrA Mutants of Escherichia coli K12. By H. NA...