This study was conducted to determine chromatin integrity and DNA damage by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and DNA damage. A significant variation was found in semen parameters after thawing. Highly sig...

in this study the effect of the alkylating agent, sulfur mustard, on calf thymus chromatin was investigated using uv/vis spectroscopy, gel electrophoresis and thermal denaturation techniques. the results show that treatment of isolated chromatin with sulfur mustard releases histones from the core particles but does not affect histone h i and nonhistone chromosomal proteins. the content of relea...

background: the particles in the range of 1-100 nm are called nanoparticles56t. gold nanoparticle is one of the most important metal nanoparticles with wide usage56t.objective: 56tthis study investigated the effects of gold nanoparticles on sperm parameters and chromatin structure in mice56t.materials and methods: 56tin this experimental study, 72 male bulb-c mice were divided into 9 groups inc...

introduction: to evaluate and compare efficiency of sil-select with percoll to recover sperm with normal chromatin in morphology and also studyeffect of these parameters on fertilization, embryo quality and cleavagescore. materials and methods: assessment of semen parameters, protamine deficiency (cma3) and excessive histone (aniline blue staining) were carried out before and after sperm proces...

In this study calf thymus chromatin was fractionated into active (S1 and S2) and inactive (P 2) chromatin. Then the interaction of an anthracyc1ine antibiotic, adriamycin, with these fractions was investigated employing absorption and difference UV Nis spectroscopy and SDS and agarose gel electrophoreses. The results suggest that the binding of adriamycin to the S 1 fraction is slight but ...

gibberellic acid (ga3) is a group of plant hormones identified in various plants. the aim of this study was to determine the effects of ga3 on sperm parameters and in vitro fertilization (ivf). fifty six adult male rats were divided into seven groups as, control, treatment and sham. following 15, 30 and 45 days of ga3 and methanol alcohol (ma) administration, rats were euthanized and epididymis...

Differentiation is an epigenetic process which is installed by changes of transcriptional programs over successive cellular divisions. A number of studies have reported the effects of biochemical modifications of chromatin (DNA and chromatin proteins) on the regulation of transcription. Although, these studies are able to explain how transcription of a given gene is regulated (toward activation...

differentiation is an epigenetic process which is installed by changes of transcriptional programs over successive cellular divisions. a number of studies have reported the effects of biochemical modifications of chromatin (dna and chromatin proteins) on the regulation of transcription. although, these studies are able to explain how transcription of a given gene is regulated (toward activation...

background: the aim of present study is to evaluate the effect of polyvinyl pyrrolidone (pvp) routinly used during icsi procedure on sperm membrane integrity, and sperm chromatin status. materials and methods: this study was carried out on 21 semen samples from the infertile men referring to isfahan fertility and infertility center. the processed semen samples were divided into two portions. on...

In this study the effect of the alkylating agent, sulfur mustard, on calf thymus chromatin was investigated using UV/Vis spectroscopy, gel electrophoresis and thermal denaturation techniques. The results show that treatment of isolated chromatin with sulfur mustard releases histones from the core particles but does not affect histone H I and nonhistone chromosomal proteins. The content of ...