OBJECTIVE The study objectives were to (1) assess the efficacy of N,O-carboxymethyl chitosan on postsurgical adhesion formation after cardiac surgery using a rabbit cardiac injury model and (2) explore the mechanism of action of N,O-carboxymethyl chitosan in the prevention of postsurgical adhesions using in vitro experimentation. METHODS In the rabbit cardiac injury model, cardiac injury was ...

Granulocyte macrophage colony stimulating factor, a potent hematopoietic cytokine, has been shown to stimulate production of white blood cells following chemotherapy. Therefore, the granulocyte macrophage colony stimulating factor gene is a potential candidate for the treatment of different pathological conditions. The purpose of this study is to investigate the suitability of chitosan as carri...

The depolymerization of chitosan by plasma in the presence of hydrogen peroxide (H(2)O(2)) was investigated. The efficiency of the depolymerization was demonstrated by means of determination of viscosity-average molecular weight and gel permeation chromatography (GPC). The structure of the depolymerized chitosan was characterized by Fourier-transform infrared spectra (FT-IR), ultraviolet spectr...

A chitosanase was purified from jelly fig latex by ammonium sulfate fractionation (50-80% saturation) and three successive column chromatography steps. The purified enzyme was almost homogeneous, as determined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and gel activity staining. The molecular mass of the enzyme was 20.5 kDa. The isoelectric point (pI) was <3.5, as estimated by isoelec...

Objective- The healing effect of chitosan supported cerium oxide nanoparticles (nano-CeO2) on cutaneous experimental excisional wound infected with pseudomonas aeruginosa was studied in rat. Design- Experimental Study. Animals- Sixty male albino rats. Procedures- The chitosan supported nano-CeO2 hydrogel was prepared and characterized using Fourier-transform infrared spectro...

background :   chitosan is unbranched polysaccharide composed of d-glucosamine and n-acetyl-d-glucosamine. chitosan, derived from shrimp shell, has broad antimicrobial properties against gram-negative, gram-positive bacteria and fungi.   methods :  chitosan was extracted from shrimp shell and studied for cell entrapment and anti-bacterial properties. the hydrogel chitosan was used as the beads ...

Chitosan can readily dissolve in a precooled aqueous alkali-urea solution, a solvent that has previously been developed to dissolve cellulose. Upon heating, the resulting solutions quickly become a gel. The thermal gelling of the chitosan solutions was studied by rheology. Initially, a temperature ramp test was used to determine the gelation temperatures (Tgel). It was found that Tgel does not ...

Chemically stable porous azacrown ether-crosslinked chitosan films were prepared by reacting varying molar amounts of N,N-diallyl-7,16-diaza-1,4,10,13-tetraoxa-dibenzo-18-crown-6 (molar equivalents ranging from 0, 0.125, 0.167, 0.25 and 0.5) with chitosan. Their chemical and structural properties were characterized by solid state-nuclear magnetic resonance (NMR), elemental, Fourier transform in...

BACKGROUND: The development of injectable sustained-release products are of great interest to veterinary pharmaceuticals and animal health business. Recently, great attention has been paid to in situ gel-forming chitosan/beta-glycerophosphate (chitosan/β-GP) solutions due to their good biodegradability and thermosensitivity. OBJECTIVES: The general aim of this study was to prepare a novel in si...