8 BACKGROUND: Chemical chitin extraction generates large amounts of wastes and increases partial 9 deacetylation of the product. Therefore, the use of biological methods for chitin extraction is an 10 interesting alternative. The effects of process conditions on enzyme assisted extraction of chitin from 11 the shrimp shells in a systematic way were the focal points of this study. 12 RESULTS: De...

ABSTRACT: The effect of methods to remove protein content on the properties glucosamine hydrochloride from shells white leg shrimp (Litopenaeus vannamei) and black tiger (Penaeus monodon) was investigated. Chitin obtained by demineralization in 6% HCl for 12h, deproteinization two different (first group soaked 8% NaOH 36h second treated Alcalase enzyme at concentration 0.2% 36h). Two samples we...

This study presents results of research on a number of microorganisms in soil of the Chełmżyńskie lake watershed and their role in the decomposition of chitin. The authors also examined the level of respiration activity of soil in the presence of shrimp waste. Results demonstrate that the number of microorganisms in soil were variable. During the entire research period, the analyzed groups of m...

Chitin is the most abundant natural biopolymer that can be converted to chitosan, which has various biomedical applications. This study was conducted to investigate the antioxidant activity of chitosan extracted from shrimp shells on body weight of broiler chickens. The study comprised three experiments. In experiment 1, shrimp (Penaeus merguiensis) shells were demineralized using HCL (1N), dep...

The culture of crustaceans, such as shrimp and crabs, in Indonesia has resulted a significant increase waste production, particularly the form shells heads. This contains valuable components like chitin, chitosan, carotenoids, which can be extracted utilized aquaculture activities. Some research results show that use derived from fish feed, is functional polysaccharide with various biological a...

Chitinous material was extracted from mycelia of Aspergillus niger and Mucor rouxii grown in yeast peptone dextrose broth for 15 and 21 days, respectively. The extracted material was characterized for purity, degree of acetylation, and crystallinity and tested for antibacterial and eliciting properties. The maximum glucosamine level determined in the mycelium of A. niger was 11.10% dw and in th...

Lactobacillus plantarum, as a potential lactic acid and protease producer, was used for biological extraction of chitin from shrimp shell. L. plantarum was grown in a batch culture containing shrimp shell powder and date syrup, incubated at 30 °C. The produced organic acids and proteases in L. plantarum culture were able to demineralize and deproteinize shrimp shell. Percentages of deproteiniza...

Three chitinase isozymes, HoChiA, HoChiB, and HoChiC, were purified from the stomach of the greenling, Hexagrammos otakii, by ammonium sulfate fractionation, followed by column chromatography on Chitopearl Basic BL-03 and CM-Toyopearl 650S. The molecular masses and pIs of HoChiA, HoChiB, and HoChiC are 62 kDa and pH 5.7, 51 kDa and pH 7.6, and 47 kDa and pH 8.8, respectively. Substrate specific...