Protein content by type and amount is an important quality criterion in the analysis of milk from various animals. This Application Note describes the analysis of milk by on-chip electrophoresis with the Agilent Protein 80 kit and the Agilent 2100 Bioanalyzer. Advantages of this method for milk protein analysis in comparison to conventional SDS-PAGE include: • Fast, reproducible and robust all ...

The paper reports the development of a microfluidic chip-capillary electrophoresis device using UV/Vis detection for profiling of urinary markers with adjustable on-chip sample dilution. A PMMA polymer microfluidic chip-CE device with on-chip ferrofluid valve control for adjustable sample dilution is developed for profiling of urinary markers related to metabolism disorders for clinical diagnos...

BACKGROUND Microalbuminuria is an important prognostic marker in diabetic nephropathy and cardiovascular disease. Initially, most commercial assays used immunoreactivity to quantify microalbuminuria; however, size-exclusion HPLC demonstrated the existence of nonimmunoreactive forms of albumin that may not be detected by immunoassay. Recent liquid chromatography tandem mass spectrometry analyses...

Avidin transgenic plants are a potential tool for providing resistance against various species of insect pests due to the sequestration of vitamin H (biotin) in the plant from the insect pests. In this project we compared three techniques for avidin determination in transgenic tobacco plants, a novel chip-based capillary electrophoretic method (Experion), classical polyacrylamide gel electropho...

Chiral separations of fluorescein isothiocyanate-labeled amino acids have been performed on a microfabricated capillary electrophoresis chip to explore the feasibility of using such devices to analyze for extinct or extant life signs in extraterrestrial environments. The test system consists of a folded electrophoresis channel (19.0 cm long x 150 microns wide x 20 microns deep) that was photoli...

In proteomics research still two-dimensional gel electrophoresis (2D-GE) is currently used for biomarker discovery. We applied free flow electrophoresis (FFE) separation technology combined with biomolecular interaction sensing using Surface Plasmon Resonance (SPR) imaging in an integrated proteomics-on-a-chip device as a proof of concept for biomarker discovery.