Three dimensional gels of native type I collagen fibres have been used as a substratum for the growth and migration of Chinese hamster ovary cells (fibroblastoid cell line) and RPMI-3460 melanoma cells (tumorigenic cell line from Syrian hamster). Quantitative data concerning the migration of these cells from the gel surface into the 3-dimensional collagen gel matrix have been obtained. The migr...

background: transient gene expression (tge) gained popularity over the last decade as a rapid method for the production of milligram to gram quantities of recombinant proteins for preclinical studies in biophama industry. thereby, the optimization of the tge technique for chinese hamster ovary (cho) as the dominant host for the production of biotherapeutics is of great interest to reach the val...

Background Seven out of the ten top-selling biopharmaceuticals in 2011 are produced in Chinese Hamster Ovary (CHO) cells [1]. This tremendous commercial interest makes the development and application of strategies for cell line optimization, like gene overexpression or knockdown to enhance cell specific productivity and cellular growth, highly interesting. In this work, we investigated the knoc...

The goals of this research were to determine: 1) the effect of continuous irradiation on Chinese Hamster Ovary cells, CHO-K1 (wild type) and xrs-6 cells (mutant), and 2) the effect of continuous irradiation under low (5%) and high (20%) ambient oxygen conditions. Other authors have found continuous irradiation at low dose rates to have beneficial effects on cells. Oxygen is known to be harmful ...

The effect of 26-hydroxycholesteroI and other intermediates in bile acid synthesis on HMG-CoA reductase activity was studied in Chinese hamster ovary (CHO) cell culture. Incubation of CHO cells for 5 hr in 0.25 PM 26-hydroxycholesterol caused a 40% inhibition of HMG-CoA reductase activity. All other intermediates tested including 3B-hydroxy-5cholenoic acid and cholest-5-ene-3B,7a,26-triol, oxid...

A new multiple drug-resistant Chinese hamster ovary cell line, ( IK )SMRs, has been isolated which demonstrates a direct correlation between reduced cellular topoisomerase II activity (5-fold reduction) and a low level of resistance (3to 7-fold) to topoisomerase II inhibitors. This cell line, initially selected for resistance to 9-(4,6-0-ethylidene-i8-D-glucopyranosyl)-4'-demethylepipodophyllot...