نتایج جستجو برای: chaperone
تعداد نتایج: 13493 فیلتر نتایج به سال:
We report on a study that combines advanced fluorescence methods with molecular dynamics simulations to cover timescales from nanoseconds milliseconds for large protein, the chaperone Hsp90.
A functional association is uncovered between the ribosome-associated trigger factor (TF) chaperone and ClpXP degradation complex. Bioinformatic analyses demonstrates conservation of close proximity tig, gene coding for TF, genes suggesting a interaction. Effect TF on ClpXP-dependent varies based nature substrate. While some substrates are slowed down or unaffected by surprisingly, increases ra...
Background and Aims: Q59L mutant of L-asparaginase enzyme from Escherichia coli (E. coli) has been introduced with lower side effects. This version of the enzyme might have potential applications in the treatment of leukemia patients. We utilized SHuffle T7 strain of E. coli, to produce the mutant enzyme in the presence of chaperone molecules. Materials and Methods: Q59LAsp gene was cloned in...
Mucopolysaccharidosis type IIIB is a devastating neurological disease caused by lack of the lysosomal enzyme, α-N-acetylglucosaminidase (NAGLU), leading to toxic accumulation heparan sulfate. Herein we explored pharmacological chaperone approach enhance residual activity NAGLU in patient fibroblasts. Capitalizing on three-dimensional structures two modest homoiminosugar-based inhibitors complex...
We performed proteomic studies using the GRP78 chaperone-inhibitor drug AR-12 (OSU-03012) as bait. Multiple additional chaperone and chaperone-associated proteins were shown to interact with AR-12, including: GRP75, HSP75, BAG2; HSP27; ULK-1; and thioredoxin. AR-12 down-regulated in situ immuno-fluorescence detection of ATP binding chaperones using antibodies directed against the NH2-termini of...
We found that Arabidopsis AtTDX, a heat-stable and plant-specific thioredoxin (Trx)-like protein, exhibits multiple functions, acting as a disulfide reductase, foldase chaperone, and holdase chaperone. The activity of AtTDX, which contains 3 tetratricopeptide repeat (TPR) domains and a Trx motif, depends on its oligomeric status. The disulfide reductase and foldase chaperone functions predomina...
Plasmids that can be used for controlled expression of the DnaK-DnaJ-GrpE and/or GroEL-GroES chaperone team were constructed in order to facilitate assessment of the effects of these chaperone teams on folding or assembly or recombinant proteins in Escherichia coli. A typical pACYC184-based plasmid which was obtained could express the major DnaK-DnaJ-GrpE and GroEL-GroES chaperone teams from se...
Using Langevin dynamics simulations, we investigate the dynamics of chaperone-assisted translocation of a flexible polymer through a nanopore. We find that increasing the binding energy ε between the chaperone and the chain and the chaperone concentration N(c) can greatly improve the translocation probability. Particularly, with increasing the chaperone concentration a maximum translocation pro...
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