Over three decades after the generation of the first mouse monoclonal antibodies by Kohler and Milstein, recombinant antibodies are the fastest growing class of therapeutic proteins. Furthermore, antibodies are key detection reagents in research and diagnostics. Technology improvements have provided several approaches to manufacturing human antibodies with high affinity for biologically relevan...

AIMS Because immunoglobulin abnormalities may affect the kidney, investigation of renal biopsies requires immunohistological study of light chains. A problem is that most antibodies to light chains react with whole immunoglobulins as well as free light chains, and there are generally many more whole immunoglobulins than free light chains. The usefulness of antibodies that only detected free lig...

after using 3 different generations of antibodies including human and non-human hyperimmune sera, monoclonal antibodies and chimeric antibodies, more recently a newer approach has been developed in which the antibody genes are cloned directly from a patient peripheral b-lymphocytes and expressed in a host like e. coli. in this study the candida albicans serotype a (nctc 3153) mannan was purifie...

beta 2 glycoprotein i (β2gpi) is a single chain 50 kda highly glycosylated glycoprotein at an approximate concentration of 4 μm in cells. the abundance of this protein in plasma and its high state of preservation indicate the important role of this protein in mammalian. in addition, β2gpi has a particular structure in the fifth domain, and is categorized as the major antigen recognized by autoa...

BACKGROUND Genomic, transcriptomic and proteomic projects often suffer from a lack of functional validation creating a strong demand for specific and versatile antibodies. Antibody phage display represents an attractive approach to select rapidly in vitro the equivalent of monoclonal antibodies, like single chain Fv antibodies, in an inexpensive and animal free way. However, so far, recombinant...

background: antiphospholipid antibodies (apl) are a heterogeneous family of anti-bodies associated with thrombosis and other complications. objective: to study the prevalence of apl in patients with thrombosis at aleppo university hospitals, syria. methods: one hundred and fifty-seven patients with venous and arterial thrombosis and 63 healthy controls were studied. anticardiolipin antibodies (...

Programmed death-ligand 1 (PD-L1) is a surface protein overexpressed in tumor cells. Recently, targeted therapy using PD-L1 antibodies to reconstitute the antitumor activity of T cells has received considerable attention as cancer treatment. Among several types anti-PD-L1 antibodies, small-sized antibody fragments are useful agents block for experimental and therapeutic purposes owing their hig...

We developed a DNA barcoding method to enable high-throughput sequencing of the cognate heavy- and light-chain pairs of the antibodies expressed by individual B cells. We used this approach to elucidate the plasmablast antibody response to influenza vaccination. We show that >75% of the rationally selected plasmablast antibodies bind and neutralize influenza, and that antibodies from clonal fam...