A member of the 270 spore germination-specific gene family in Dictyostelium discoideum is shown to encode a cellulase (endo-(1,4)-beta-D-glucanase, EC 3.2.1.4) activity. The 270-6 deduced protein shows 38% identity and 58% similarity to an avocado (Persea americana) cellulase. During spore germination in Dictyostelium extracellular cellulase activity starts to accumulate coincident with the app...

Polysomal RNA was isolated from pea epicotyls treated with (2,4-dichlorophenoxy)acetic acid, and fractionated on oligo(dT)-CELLULOSE TO YIELD POLY(A)-containing RNA. This RNA fraction was translated in a wheat embryo cell-free system and found to have more than 90% of the messenger activity in total polysomal RNA. Immunoprecipitation of the translation product by monospecific antibodies to pea ...

The substrate specificities of three cellulases and a beta-glucosidase purified from Thermoascus aurantiacus were examined. All three cellulases partially degraded native cellulose. Cellulase I, but not cellulase II and cellulase III, readily hydrolyzed the mixed beta-1,3; beta-1,6-polysaccharides such as carboxymethyl-pachyman, yeast glucan and laminarin. Both cellulase I and the beta-glucosid...

It is possible to improve cellulase production by controlling fungal morphology. Farnesol, the first quorum-sensing molecule found in eukaryotic organisms, is reported to influence the morphology of fungi. In this work, farnesol was investigated for its effect on morphology and cellulase production of Penicillium decumbens. Scanning electron microscopy (SEM) revealed that farnesol promoted the ...

Cellulase induction by beta-glucodisaccharides was investigated by using non-cellulase-induced mycelia of Penicillium purpurogenum P-26, a highly-cellulase-producing fungus. Gentiobiose induced significant amounts of cellulase compared with cellobiose when nojirimycin was added to the induction medium to inhibit extracellular beta-glucosidase activity. Thiogentiobiose (6-S-beta-d-glucopyranosyl...

Cellulase (beta-1,4-glucan-glucanohydrolase EC 3.2.1.4) activity increased during abscission and was localized in the cell separation layer of Phaseolus vulgaris L. cv. Red Kidney (bean), Gossypium hirsutum L. cv. Acala 4-42 (Cotton) and Coleus blumei Benth. Princeton strain (Coleus) abscission zone explants. Cellulase activity was optimum at pH 7, was reduced by one-half after heating to 55 de...

The aim of the present work was focused on the cellulase production by Aspergillus niger using different substrates (i.e) rice bran, wheat bran, black gram bran, coconut oil cake, groundnut oil cake, and gingely oil cake. Solid state fermentation holds tremendous potentials for the production of the enzyme cellulase by Aspergillus niger. These agro industrial residues are cheap raw materials fo...

Cellulase-producing bacteria were isolated from soil and identified as Pseudomonas fluorescens, Bacillus subtilIs, E. coli, and Serratia marcescens. Optimization of the fermentation medium for maximum cellulase production was carried out. The culture conditions like pH, temperature, carbon sources, and nitrogen sources were optimized. The optimum conditions found for cellulase production were 4...

Bioethanol from cellulosic raw material has proved to be the best alternative renewable energy source. Cellulase is a multienzyme complex catalyses the bioconversion of cellulose to glucose, which can be used for ethanol production. The objective of this research is to reduce the cost of cellulase production by optimization of fermentation conditions and modeling of the fermentation process. Re...

The physiology and anatomy of abscission has been studied in considerable detail; however, information on the regulation of gene expression in abscission has been limited because of a lack of probes for specific genes. We have identified and sequenced a 595 nucleotide bean (Phaseolus vulgaris cv Red Kidney) abscission cellulase cDNA clone (pBACl). The bean cellulase cDNA has extensive nucleic a...