background: rna interference (rnai) is the mechanism of gene silencing-mediated messenger rna degradation by small interference rna (sirna), which becomes a powerful tool for in vivo research, especially in the areas of cancer. in this research, the potential use of an expression vector as a specific sirna producing tool for silencing of bcr-abl in k562 cell line has been investigated. methods:...

expression of receptor tyrosine kinase ror1 in a wide variety of cancers has emerged as a new era focusing on targeting this receptor in cancer therapy. our preliminary re-sults indicate the presence of a truncated transcript of ror1 in tumor cells. the trun-cated ror1 encompasses extracellular and transmembrane domains, lacking catalytic kinase domain (ror1-ecd). as enzyme activity is highly d...

background: p19 cells are mouse embryonic carcinoma cells which contain pluripotent ability, like stem cells, to differentiate into different cell lines. there are several properties for this cell line that make it a valuable cell model for study of developmental stages. methods: at the first step, pts2-egfp coding sequence which was cloned in pucd2.hygro vector was used for transfection in to ...

enterohemorrhagic escherichia coli (ehec), such as e. coli o157:h7, are emerging food-borne pathogens worldwide. this micro-organism can damage the epithelial tissue of the large intestine. the cytotoxic effects can be neutralized by probiotics such as bifidobacterium bifidum. probiotics are viable cells that have beneficial effects on the health of the host. the preventing activity of b. bifid...

objective(s): to investigate the efficacy of interleukin 11 (il-11) towards the high dose methotrexate (hdmtx)-concurrent rat small intestinal mucositis and its impacts on the proliferation of the human t-lymphoblastic leukemia (cem) cell line. materials and methods:95 wistar rats were randomly divided into five groups, the normal control group (a), the methotrexate (mtx) control group (b), the...

background and objective: the management of apoptotic cell death has been considered as a putative therapeutic strategy for cancer treatment. in the present study we investigated the putative pro-apoptotic effect of allicin, the main garlic organosulfur component with repeatedly claimed chemopreventive potency, on the human adenocarcinoma cell line ht29 as an apoptosis resistant cell line, in v...

the cytotoxicity of the biomaterials is a key issue that should be addressed prior to pre-clinical applications. this study was designed to evaluate and compare the cytotoxixity of two forms of bioactive glasses:nanopowder:andmicropowder. human hgf1-pi53 gingival fibroblast cells were used to evaluate the cytotoxicity of 0.5, 1, 1.5, 2, 5, 10, 15 and 20 mg/ml concentrations of the two bioactive...

background: pattern recognition receptors (prrs) are the main sensors of pathogen and danger signals in innate immunity of which toll like receptors (tlrs) are the most studied ones. the contribution of prrs in cerebral inflammation induced by microbial infection, tissue damage and cancer has not extensively been addressed so far. glioma is the most common tumor of the central nervous system an...

background: interfering with cell proliferation and survival is a critical role for antineoplastic drugs leading to cell death through induction of apoptosis. alternative treatments with herbal extracts offer insights into acute myeloid leukemia (aml) therapy. parthenolide (ptl), an extract from feverfew, induces apoptosis in primary human leukemia stem cells (lscs) and bulk leukemic cell popul...