objective: we introduce an rgd (arg-gly-asp)-containing peptide of collagen iv origin that possesses potent cell adhesion and proliferation properties. materials and methods: in this experimental study, the peptide was immobilized on an electrospun nanofibrous polycaprolactone/gelatin (pcl/gel) hybrid scaffold by a chemical bonding approach to improve cell adhesion properties of the scaffold. a...

Immobilization of brewing yeast onto a cellulose-based carrier obtained from spent grains, a brewing byproduct, by acid/base treatment has been studied in a continuously operating bubble-column reactor. The aim of this work was to study the mechanisms of brewing yeast immobilization onto spent grain particles through the information on physicochemical surface properties of brewing yeast and spe...

Surface modification by functional groups promotes endothelialization in biohybrid artificial lungs, but whether it affects endothelial cell stability under fluid shear stress, and the release of anti-thrombotic factors, e.g. nitric oxide (NO), is unknown. We aimed to test whether surface-modified silicone tubes containing different functional groups, but similar wettability, improve collagen i...

Immobilization is the method of entrapping/attaching the microbial or plant cells in a suitable matrix. Different methods such as encapsulation, gel entrapment, covalent bonding, cross linking and adsorption is carried out to prepare immobilized cells. When compared to enzyme immobilization, cell immobilization is regarded as a convenient method because of its low cost and improved stability. T...

objective: in this study we introduced an rgd-containing peptide of collagen iv origin that possesses potent cell adhesion and proliferation properties. this peptide was immobilized on a nanofibrous polycaprolactone/gelatin scaffold after which we analyzed human bone marrow-derived mesenchymal stem cells (hbmscs) adhesion and proliferation on this peptide-modified scaffold. methods: nanofibrous...

presently there are a number of fine and sensitive methods for determining chemical or biochemical substance among which bio electrodes represent the most recent development. bioelectrodes consist of biologically active materials (e.g enzyme, antibody, whole cell or cell fragments) held in close proximity to a suitable electrochemical transducer that sense specific electroactive enzyme substrat...

We previously reported that short-term immobilization stress of rats causes increased colonic mucin release, goblet cell depletion, prostaglandin E2 secretion, and colonic mast cell activation, as well as increased colonic motility. The purpose of this study was to investigate whether neurotensin (NT), a peptide expressed in both brain and digestive tract, participates in these responses. Rats ...

Techniques of immobilization of microbial- cells have been established by multifunctional reagents such as glutaraldehyed on a solid support. Immobilized cell reactor was designed to demonstrate stability of cross linking of whole cells for the production of organic acids. An eighty-six percent conversion of glucose was obtained for an eight hour retention time. The immobilized cell reacto...

Presently there are a number of fine and sensitive methods for determining chemical or biochemical substance among which bio electrodes represent the most recent development. Bioelectrodes consist of biologically active materials (e.g enzyme, antibody, whole cell or cell fragments) held in close proximity to a suitable electrochemical transducer that sense specific electroactive enzyme subs...