The cell counting process is an important procedure for various and cell-related research applications. Many life science-related studies examine the cells to compare results concerning numbers variations. Most of related are conducted using manual methods. However, difficult, time-consuming, fallible. This study proposes automated software computer vision (CV) technology experimental investiga...

introduction: mineral trioxide aggregate is being widely used for root end filling, pulp capping, perforation repair, and other endodontic procedures. recently, a material similar to proroot mineral trioxide aggregate (pmta) was developed in iran named root mineral trioxide aggregate (rmta) with the claim of having the exact result of original mta. the purpose of this study was to compare the a...

Computer cell counting experiments were performed in order to examine the consequences of over- or under-counting. The three-dimensional reaggregate culture laboratory environment for cell counting was used as a model for computer simulation. The laboratory environment for aggregate and cell sizes, numbers and spatial placement in gelatin blocks was mimicked in the computer setup. However, in t...

Counting cells and colonies is an integral part of high-throughput screens and quantitative cellular assays. Due to its subjective and time-intensive nature, manual counting has hindered the adoption of cellular assays such as tumor spheroid formation in high-throughput screens. The objective of this study was to develop an automated method for quick and reliable counting of cells and colonies ...

This paper describes a part of current research work on counting dead and live hepatocytes (liver cells) in cultures from microscopic images. The requirement of the work is to develop an automatic cell counting process that is simple, fast, and achieves high level of count accuracy. Cells in the acquired images are difficult to identify due to low contrast, uneven illumination, gray intensity v...

Objective: The application of human cord blood (hCB) is limited to children by using relatively small volume of cord blood that does not contain enough hematopoietic stem cells (HSCs). So, efforts for applying cord blood stem cells in transplantation have led to establishment of some approaches for ex vivo expansion of HSCs such as garcinol. Materials and Methods: CD133+ HSCs were separated by ...