Cefquinome is a cephalosporin with broad-spectrum antibacterial activity, including activity against enteric Gram-negative bacilli such as Escherichia coli. We utilized a neutropenic mouse model of colibacillosis to examine the pharmacodynamic (PD) characteristics of cefquinome, as measured by organism number in homogenized thigh cultures after 24 h of therapy. Serum drug levels following 4-fol...

BACKGROUND In order to provide some basis for effective dosage regimens that optimize efficacy with respect to bacteriological and clinical cures, the in vivo activity of cefquinome against a clinical Escherichia coli (E.coli) strain (the minimum inhibitory concentration value for this strain equals to the MIC90 value of 0.25 μg/ml for 210 E.coli strains isolated from pigs) was investigated by ...

Stability of ceftiofur sodium and cefquinome sulphate in intravenous solutions was studied. Chromatographic separation and quantitative determination were performed by using a high-performance liquid chromatography with UV-DAD detection. During the stability study, poly(vinylchloride) minibags were filled with a solution containing 5 mg of ceftiofur sodium or cefquinome sulphate and diluted to ...

The degradation behavior of cefquinome sulphate in alkaline medium at different temperatures was investigated using both first derivative spectrophotometric and HPLC methods. The drug degradation was found to be pH and temperature dependant. The pH-rate profile indicated a first order dependence of Kobs on [OH(-)] at pHs ranging between 9 and 11. Arrhenius plot obtained at pH 10 was linear betw...

The objective of the present study was to determine whether there was an association between the in vitro antimicrobial susceptibility test results of subclinical mastitis pathogens and bacteriological outcomes of intramammary treatments using cefquinome. A total of 110 intramammary pathogens from 51 cows were assessed in this study. Most intramammary infections were due to coagulase-negative s...

BACKGROUND Mutant prevention concentration (MPC) is an alternative pharmacodynamic parameter that has been used to measure antimicrobial activity and represents the propensities of antimicrobial agents to select resistant mutants. The concentration range between minimum inhibitory concentration (MIC) and MPC is defined as mutant selection window (MSW). The MPC and MSW parameters represent the a...

Cefuroxime sodium is a second-generation parentally administered cephalosporin, used in both human and veterinary medicine. In veterinary medicine, cefuroxime sodium, active on Streptococcus agalactiae, Streptococcus dysgalactiae, is administered intramammary for the treatment of mastitis in cattle during the lactating period. Cefquinome sulphate is a fifth generation cephalosporin, which is ad...

This manuscript presents a developed method for determining cefquinome sulfate in piglets blood plasma intended clinical and pharmaceutical research of veterinary drugs based on it. Blood proteins were precipitated twice with solution trichloroacetic acid. The supernatant was further purified by series solid-phase extraction. Separation performed an inverted phase Kinetex EVO С18 column using a...

This work investigates the pharmacodynamic effectiveness of cefquinome against environmental Escherichia coli mastitis infection, following an intramammary administration. We established the pharmacokinetic and pharmacodynamic (PK/PD) model in lactating mice. The PK/PD parameters were identified to achieve an antibacterial efficacy as indicated by PD activity, cytokine expression and PK/PD simu...