We have investigated the chromatin structure of wild-type and mutationally altered centromere sequences in the yeast Saccharomyces cerevisiae by using an indirect end-labeling mapping strategy. Wild-type centromere DNA from chromosome III (CEN3) exhibits a nuclease-resistant chromatin structure 220-250 base pairs long, centered around the conserved centromere DNA element (CDE) III. A point muta...

Clathrin independent endocytosis (CIE) is a form of endocytosis present in all cells that mediates the entry of nutrients, macromolecules and membrane proteins into cells. When compared to clathrin-dependent endocytosis (CDE), however, much less is known about the machinery involved in forming CIE endosomes. One way to distinguish CIE from CDE has been to deplete cells of coat proteins involved...

Bronchial hyperreactivity (BHR) is associated with the presence of airway inflammation in asthma and is seen in individuals occupationally exposed to grain dust. To better understand the relationship between BHR and pulmonary inflammation after grain dust exposure, we carried out an inhalation challenge to corn dust extract (CDE) on seven subjects with BHR [a 20% or greater decrease in forced e...

INTRODUCTION The main treatment of burns is early excision of injured tissues. AIM TO COMPARE TWO DIFFERENT METHODS OF EXAMINATION OF BURNED PATIENTS: clinical burn depth examination (CDE) and laser Doppler imaging (LDI). MATERIAL AND METHODS A prospective randomized study of 57 burn patients treated in 2009-2011 was carried out. The burned patients were randomized into a CDE group and an L...

Science projects are increasingly investing in computational reproducibility. Constructing software pipelines to demonstrate reproducibility is also becoming increasingly common. To aid the process of constructing pipelines, science project members often adopt reproducible methods and tools. CDE is a software packaging tool for reproducible research that encapsulates source code, datasets and e...

Color histogram is an important technique for color image database indexing and retrieving. However, the main problem with color histogram indexing is that it does not take the color spatial distribution into consideration. Previous researches have proved that the effectiveness of image retrieval increases when spatial feature of colors is included in image retrieval. In this paper, two new des...

We investigated the structural requirements of the centromere from chromosome III (CEN3) of Saccharomyces cerevisiae by analyzing the ability of chromosomes with CEN3 mutations to segregate properly during meiosis. We analyzed diploid cells in which one or both copies of chromosome III carry a mutant centromere in place of the wild-type centromere and found that some alterations in the length, ...

OBJECTIVE Intranasal application of cowshed dust extract (CDE) during sensitisation in a murine model of experimental asthma leads to a significant alleviation of the clinical parameters of the allergic immune response. However, neither the immunological mechanisms underlying this protective effect nor all of the protective substances included in CDE have yet been described. Recently, complemen...

By R. R. RACE, M.R.C.S., L.R.C.P., A. E. MOURANT, B.M., B.Ch., MA., D. Phil., SYLVIA D. LAWLER, M.W, B.S., and RUTH SANGER, B.Sc.* T HIS paper reports the results of testing 1073 samples of blood, from English people, with the Rh antibodies anti-C, anti-C”, anti-c, anti-D, anti-E and anti-c. To these are added another 92.7, tested with anti-C, anti-c, anti-D and antiE, the results of which have...