Selective T-cell depletion prior to cell or organ transplantation is considered a preconditioning regimen induce tolerance and immunosuppression. An immunotoxin consisting of recombinant anti-CD3 antibody conjugated with diphtheria toxin was used eliminate T-cells. It showed significant activity in the peripheral blood lymph nodes animal models previous studies. To date, comprehensive evaluatio...

Abstract Objective: In vitro cytokine production by peripheral blood mononuclear cells (PBMCs) is an important and reliable measure of immunocompetence. PBMC can be stimulated directly after isolation or frozen for later use. However, cryopreservation may affect cell recovery, viability functionality. This study aims to investigate synthesis in ex-vivo fresh cryopreserved CD4+ CD4- T cells. Met...

We examined 23 patients aged 40 to 65 years (mean age 54.526.72) with a diagnosis of acute coronary syndrome (ACS) at admission, who underwent emergency or delayed stenting day later. All had arterial hypertension as concomitant disease. Upon additional examination, blood troponin levels were determined, ECG was performed in the time dynamics. Acute myocardial infarction ST elevation diagnosed ...

This study investigates the potential of the alpha CD3-induced killer cells for use in adoptive immunotherapy of tumor growth. The alpha CD3-induced, activated, killer cells (CD3-AK) were generated in DBA/2 (H-2d) splenocytes by preactivation with alpha CD3 and were then cultured in the presence (CD3-AK [alpha CD3+]) or absence (CD3-AK [alpha CD3-]) of alpha CD3. The conventional lymphokine-act...

Background: Androgenic hair loss (AGA) and alopecia areata (AA) are common conditions which sometimes are histologically difficult to differentiate. This study was conducted to detect differentiating features of these two disorders with IHC analysis of T-cell subsets in the inflammatory infiltrates of alopecia areata and androgenetic alopecia. Methods: This cr...

BACKGROUNDS: Seminoma is frequently observed in human and canine testes especially in cryptorchids. Canine and human seminomas are typically associated with leukocytic infiltration. OBJECTIVES: We aimed to identify the type of lymphocytes that infiltrate to seminomas. METHODS: Tumor infiltrating lymphocytes were evaluated by immunohistological techniques in 5 dogs with diffuse seminoma. Routine...

Women with immunodeficiency conditions are a separate cohort of patients who need the close attention doctors and social workers. The aim study was to determine distribution CD68 CD3 immune cells in cervix women infectious non-infectious immunodeficiency, namely, HIV infection, chronic alcoholism their combination. Research groups were formed based on results autopsies 100 reproductive age, 25 ...

Among all histological types, serous carcinomas account for up to 85%. Due pronounced heterogeneity (at the molecular and genetic level) chemoresistance, difficulties arise in finding active targets tumor elimination.
 Aim. To establish a link between population composition of tumor-associated immune cells microenvironment stage ovarian cancer.
 Materials methods. The analysis patholo...

behcet disease (bd) is a systemic inflammatory disease of the unknown etiology. there is however, some evidence to suggest that immunological abnormalities are important in its pathogenesis, furthermore several t-cell abnormalities which may be quite relevant to autoimmune origin of the disease have been described. we report here our study of t-cell subsets, b and nk cells in 68 patients with b...

Infertile marriage is a complex socio-economic problem; the frequency of infertility in Ukraine 17-20%, which prompts further study its mechanisms, including immune ones, order to develop appropriate methods correction. Purpose - characterize phenotypic features T-cells and their subpopulation T-helpers (CD3+CD4+) with expression intracellular cytokines women history at different stages pregnan...