Human leukemia cells are being regularly grown intraperitoneally in the intact newborn Syrian hamster as 3 serially transplantable sublines of lymphosarcoma. These sublines, established from suspension cultures of human leukemic lymphoblasts (CCRF-CEM cells) by the implantation of large numbers of cells, exhibit similar transplantation and growth characteristics, and are presently in their llth...

The growth of human CCRF-CEM T-cell lymphoblastic leukemia was studied in mice immune deprived by different techniques, and in CD-nu/nu athymic mice. Female CBA/CaJ mice were immune deprived by infant thymectomy, priming with 1-beta-D-arabinofuranosylcytosine (200 mg/kg) 48 h prior to total body irradiation (925 cGy) designated theta ara-C gamma; or after thymectomy the mice received 925 cGy to...

Chem. 166:397, 1946), which requires biotin, pantothenate (or ,B-alanine), and NA for optimal growth; thiamine is only stimulatory. Pantothenate (or $-alanine) stimulates but is not essential for the growth of CCRF no. 60705. m-Inositol (2.0 ,g/ml) and pyridoxine (2.0 ,g/ml) are only stimulatory, as was the case with T. cremoris ATCC no. 2512. Other vitamins, purines, pyrimidines, etc., had no ...

A new single-cell microarray chip was designed and developed to separate and analyze single adherent and non-adherent cancer cells. The single-cell microarray chip is made of polystyrene with over 60,000 microchambers of 10 different size patterns (31-40 µm upper diameter, 11-20 µm lower diameter). A drop of suspension of adherent carcinoma (NCI-H1650) and non-adherent leukocyte (CCRF-CEM) cell...

BACKGROUND Cancer cells rapidly acquire resistance leading to treatment failures. In the present study, we have evaluated the cytotoxicity of 17 methanol extracts from 11 Cameroonian medicinal plants against the sensitive leukemia CCRF-CEM cells and the best ones were further tested on a panel of 8 other human cancer cell lines, including various MDR phenotypes as well as against the normal AML...

Aptamers have emerged as promising molecular probes for in vivo cancer imaging, but the reported "always-on" aptamer probes remain problematic because of high background and limited contrast. To address this problem, we designed an activatable aptamer probe (AAP) targeting membrane proteins of living cancer cells and achieved contrast-enhanced cancer visualization inside mice. The AAP displayed...

J Can Chiropr Assoc 2014; 58(3) 1 Professor and Canada Research Chair in Spinal Function, Faculty of Rehabilitation Medicine, University of Alberta 2 Assistant Professor, Department of Human Health and Nutritional Sciences, University of Guelph 3 Assistant Professor and CCRF Chair in Spine Mechanics and Neurophysiology, Human Health and Nutritional Science, University of Guelph 4 Assistant Prof...

Two methotrexate-resistant sublines, CCRF-CEM R3/7 and CCRF-CEM R30/6, were selected from the human leukemia T-lymphoblast cell line, CCRF-CEM, after repeated exposures (7 and 6 times, respectively) for 24 h to constant concentrations (3 and 30 microM) of the drug. Analysis of the mechanism of resistance revealed no differences in levels of dihydrofolate reductase activity, its binding affinity...

Abstract. The conical–conical reflectance factor (CCRF) has been calculated as an alternative to the bidirectional distribution function (BRDF) for three types of bare sea ice with varying surface roughness (?= 0.1–10) and thicknesses (50–2000 cm) over incident solar irradiance wavelength range 300–1400 nm. comprehensive study CCRF presented here is paramount interpreting measurements from sate...

Nitrobenzylthioinosine, dilazep, and dipyridamole are potent inhibitors of equilibrative transport of nucleosides that may have pharmacological applications in modulating the therapeutic index of nucleoside antimetabolites used in cancer chemotherapy. We have compared the relative abilities of these inhibitors to reduce the toxicity of in vitro exposures to tubercidin against clonogenic progeni...