Catalase plays a major role in cellular antioxidant defense by decomposing hydrogen peroxide, thereby preventing the generation of hydroxyl radical by the Fenton reaction. The degree of catalase deficiency in acatalasemic and hypocatalasemic mice varies from tissue to tissue. They therefore may not be suitable for studying the function of this enzyme in certain models of oxidant-mediated tissue...

trichosanthes kirilowii, an important economic plant in china, is sensitive to chilling. to investigate the effect of abscisic acid (aba) treatment on the chilling tolerance of trichosanthes kirilowii maxim, different concentrations of aba were sprayed on trichosanthes kirilowii leaves and the resulting catalase (cat) and superoxide dismutase (sod) activities and the malondialdehyde (mda) conte...

Vibrio vulnificus is a virulent human pathogen causing gastroenteritis and possibly life threatening septicemia in patients. Most V. vulnificus are catalase positive and can deactivate peroxides, thus allowing them to survive within the host. In the study presented here, a catalase from V. vulnificus (CAT-Vv) was purified to homogeneity after expression in Escherichia coli. The kinetics and fun...

Objective To evaluate the effect of ubiquinone (Coenzyme Q10) and combined antioxidant therapy (CAT) on oxidative stress markers in non-proliferative diabetic retinopathy (NPDR) under clinical management. Study design In a randomized, double-blind, phase IIa, placebo-controlled, clinical trial, three study groups were formed and administered medications as follows: Group 1, Coenzyme Q10; Group ...

Catalase (CAT, EC 1.11.1.6) activity was measured in flesh tissue of six apple cultivars (Malus domestica Borkh. cvs. Braeburn, Gala, Jonagold, McIntosh, Red Delicious, and Spartan). Activity of CAT was determined for fresh and frozen tissue of the same fruit. Freezing resulted in reductions of 50 to 90% in CAT activity compared with the activity measured in crude extracts from fresh tissues. T...

Antioxidant enzymes seem to play roles in controlling the luteal function and the luteolytic action of prostaglandin F2α (PGF). The aim of this study was to clarify the roles of catalase (CAT) and glutathione peroxidase (GPx) in the luteolytic action of PGF in both corpus luteum (CL) and cultured luteal cells. Corpora lutea were collected at the early (days 2-3), developing (days 5-6), mid (day...

The crystallization and structural characterization of bovine liver catalase (BLC) has been intensively studied for decades. Forms I and II of BLC have previously been fully characterized using single-crystal X-ray diffraction. Form III has previously been analyzed by electron microscopy, but owing to the thinness of this crystal form an X-ray crystal structure had not been determined. Here, th...

Staphylococcus aureus strains without catalase activity are rare, challenging to identify with conventional biochemical methods, and, despite a supposed decreased pathogenicity, can still cause disease. The first whole-genome sequence of a catalase-negative S. aureus isolate causing severe recurrent invasive infection with two novel missense mutations in the katA gene is reported here.

In this study, chitosan polymer was modified in two different ways and used for the immobilization of bovine liver catalase (CAT). First, it activated with glutaraldehyde (GAL), then covalently immobilized onto support via lysine amino acid residue CAT. second modification, GAL-activated interacted polylysine (pLYS), reactivated GAL CAT immobilization. Samples bare (Chi), (ChiGAL), polylysine-m...

A hydrocarbon degrading Aspergillus terreus MTCC 6324 produces a high level of extremely active and stable cellular large catalase (CAT) during growth on n-hexadecane to combat the oxidative stress caused by the hydrocarbon degrading metabolic machinery inside the cell. A 160-fold purification with specific activity of around 66 × 10(5) U mg(-1) protein was achieved. The native protein molecula...