Glioma is one of the most common malignant tumors affecting the central nervous system. We screened active drugs from 12 classes to provide experimental data for the study of human glioma. Serum-free suspension clone formation was used to induce the formation of glioma stem‑like cells (GSLCs) from U251 cells. We screened active drugs from 12 class...

BACKGROUND In addition to recognized antimalarial effects, Artemisia annua L. (Qinghao) possesses anticancer properties. The underlying mechanisms of this activity are unknown. The aim of our experiments was to investigate the effects of distinct types of compounds isolated from A. annua on the immune-activated production of major mediators of angiogenesis playing a crucial role in growth of tu...

Classical homeopathy is based on the therapeutic application of highly diluted homeopathic stocks. The indications of such medicines are determined by proving, i.e. by applying the remedies in healthy subjects. However, there are several complex homeopathic medicinal products on the market with approved therapeutic indications. The efficacy of these medicines has been assessed in clinical trial...

This study aimed to investigate the anti-angiogenic activity of Vitex agnus castus methanol extract in vivo. Eggs were incubated for three days, small whole made on the fine pinpoint, next day the egg’s sac penetrated and a small frame was made in the shell. The window was resealed and eggs were returned to the incubator until day 10 of chick embryo development, 20 µl of 500mg/ml of the methano...

Semisynthesis of 5,3'-dihydroxy-3,6,7,8,4'-pentamethoxyflavone (1), a natural flavone that binds with high affinity to tubulin, was performed from hesperidin, the very abundant Citrus flavanone, by a five-step sequence. The last step of the synthesis also gave rise to 5,3'-dihydroxy-3,6,7,4'-tetramethoxyflavone (= casticin or vitexicarpin) (10), 5,3'-dihydroxy-3,7,8,4'-tetramethoxyflavone (= go...

this study aimed to investigate the anti-angiogenic activity of vitex agnus castus methanol extract in vivo. eggs were incubated for three days, small whole made on the fine pinpoint, next day the egg’s sac penetrated and a small frame was made in the shell. the window was resealed and eggs were returned to the incubator until day 10 of chick embryo development, 20 µl of 500mg/ml of the methano...