Extracts from pods and leaves of carob (Ceratonia siliqua L.) were tested for their ability to inhibit cell proliferation of mouse hepatocellular carcinoma cell line (T1). The two extracts showed a marked alteration of T1 cell proliferation in a dose-related fashion reaching the maximal effect at 1 mg/ml. Moreover, we demonstrated that leaf and pod extracts were able to induce apoptosis in T1 c...

OBJECTIVE This study was undertaken to investigate the anti-inflammatory role of fennel, carob, doum and mixture of them in improving the renal dysfunction induced in rats by the cyclosporine. DESIGN Sixty female albino rats were divided into six groups: healthy control rats, cyclosporine injected rats (positive control), other groups were injected by cyclosporine for 7 days and then diet was...

This study investigated the role of CBM35 from Clostridium thermocellum (CtCBM35) in polysaccharide recognition. CtCBM35 was cloned into pET28a (+) vector with an engineered His6 tag and expressed in Escherichia coli BL21 (DE3) cells. A homogenous 15 kDa protein was purified by immobilized metal ion chromatography (IMAC). Ligand binding analysis of CtCBM35 was carried out by affinity electropho...

There is a growing interest worldwide to find out new and cheap carbohydrate sources for production of bioethanol. In this work, carob pod (Ceratonia Siliqua) is proposed as an economical source for ethanol production, especially, in arid regions. The carob tree is an evergreen shrub native to the Mediterranean region, cultivated for its edible seed pods and it is currently being reemphasised a...

UNLABELLED Carob germ proteins have been shown to have functional properties similar to wheat gluten enabling formulation and production of yeast leavened gluten-free baked goods from a true dough rather than a stiff batter. The purpose of this research was to optimize the production of wheat-free bread containing carob germ flour, corn starch, NaCl, sucrose, hydroxypropyl methylcellulose (HPMC...

In this experiment the effect of dietary carob pods in the growth performance of fattening pigs and their meat quality, including steak chemical composition and fatty acid profile, were examined. A total of 160 weaning piglets, 30 days old, were allocated into four equal groups with 4 subgroups of 5 female and 5 males each. The animals were fed with isocaloric and isonitrogenous diets, containi...