Lignin biodegradation is catalyzed in part by ligninases, also known as lignin peroxidases (1-4). We have cloned and sequenced the gene encoding ligninase isozyme H8 from the white-rot fungus Phanerochaete chrysosporium. The gene is interrupted by eight introns, ranging in size from 49 to 69 bp, which are distributed throughout the gene. Putative transcriptional control signals (underlined) inc...

In the regulation of gene expression, particularly abiotic-stress tolerance characteristics in plants, inducible promoters play an essential role ensuring endurance and productivity crops. several previous studies, ZmDREB2.7 some DNA polymorphisms its promoter have been shown to enhance drought traits maize (Zea mays L.). With goal creating a strong drought-tolerant region derived from maize, t...

This paper presents an effective core-promoter prediction system on human DNA sequence. The system, named PromSearch, employs a hybrid approach which combines search-by-content method and search-by-signal method. Global statistics of promoter-specific contents are included to represent new significant information underlying the proximal and downstream region around transcription start site (TSS...

Adipose tissue secretes a plethora of adipokines as evidenced by characterization of subcutaneous and visceral adipose tissue secretomes. However, adipose tissue composition and secretion pattern is depot and disease dependent, influencing the adipose tissue secretome. We investigated the secretome of cancer-associated adipose tissue (CAAT) explants from breast cancer patients and explored its ...

Prohormone convertases are involved in the tissue-specific endoproteolytic processing of prohormones and neuropeptide precursors within the secretory pathway. In the present study, we have isolated genomic clones comprising the 5'-terminal region of the human prohormone convertase 2 (PC2) gene and established characteristics of the PC2 promoter region. The proximal promoter region is very G+C-r...

The promoter region of the human prostaglandin F2alpha receptor (FP) gene was isolated, sequenced, and characterized. The 5'-flanking region has minimal homology to the bovine, mouse, and rat FP promoters with the exception of a 100-bp region. The human promoter similarly lacks a canonical TATA-box and a CAAT-box. Potential binding sites for SP-1, GATA-1, STAT-1, and AP-1 are present in the 5'-...

In addition to readily identifiable TATA and CAAT boxes, chicken H1 histone genes contain a highly conserved G-rich region at about minus 80 and an A-rich H1 histone gene-specific motif some 120 bases upstream from the H1 mRNA cap site. The level of transcription from wild-type, 'G-box' and 'A-box' H1 deletion mutant templates was tested in Xenopus oocytes and in HeLa cells. Removal of the H1 g...

We present here a fast and sensitive method designed to isolate short nucleotide sequences which have non-random statistical properties and may thus be biologically active. It is based on a first order Markov analysis and allows us to detect statistically significant sequence motifs from six to ten nucleotides long which are significantly shared (or avoided) in the sequences under investigation...

A genomic clone, encompassing the 5' flanking region and the first seven exons of rat ATP citrate lyase gene, was isolated from a rat genomic library and sequenced. Primer-extension analysis showed that mRNA is transcribed at 4407 nucleotides upstream from the translation start site. Primer-extension analysis and sequencing of ATP citrate lyase cDNA amplified by PCR showed that the promoter use...