We have established that UvrABC nuclease is equally efficient in cutting mitomycin C (MC)-DNA monoadducts formed at different sequences and that the degree of UvrABC cutting represents the extent of drug-DNA bonding. Using this method we determined the effect of C5 cytosine methylation on the DNA monoalkylation by MC and the related analogues N-methyl-7-methoxyaziridinomitosene (MS-NMA) and 10-...

Cancer cells often have a high demand for antiapoptotic proteins in order to resist programmed cell death. CDK9 inhibition selectively targets survival proteins and reinstates apoptosis in cancer cells. We designed a series of 4-thiazol-2-anilinopyrimidine derivatives with functional groups attached to the C5-position of the pyrimidine or to the C4-thiazol moiety and investigated their effects ...

Based on a previous report that substituted 2-acetylphenols may be promising leads for the design of novel monoamine oxidase (MAO) inhibitors, a series of C5-substituted 2-acetylphenol analogs (15) and related compounds (two) were synthesized and evaluated as inhibitors of human MAO-A and MAO-B. Generally, the study compounds exhibited inhibitory activities against both MAO-A and MAO-B, with se...

Pathogenic strains of Escherichia coli and Salmonella enterica modify the tricatecholic siderophore enterobactin (Ent) by glucosylation of three aryl carbon atoms, a process controlled by the iroA locus [Hantke, K., Nicholson, G., Rabsch, W. & Winkelmann, G. (2003) Proc. Natl. Acad. Sci. USA 100, 3677-3682]. Here, we report the purification of the IroB protein and its characterization as the En...

Background. Postoperative C5 motor palsy is known as a common complication not only after cervical laminectomy but also after anterior discectomy. There is no consensus to verify any of the proposed mechanisms of C5 palsy following posterior cervical decompression. The authors of this article have found that C5 palsy is more common among patients with smaller C5 root exit foramen area. The purp...

In this article, the synthesis, characterization and catalytic performance of three MgAPO-11 catalysts with distinct crystal morphologies (sunflower, ball candy) are presented. Among samples, candy-like MgAPO-11-C, high crystallinity uniform particle size (of about 1 µm), was synthesized for first time by using a unique two-step dry gel method. Despite similar acid strength different resulted i...

The aim of this work was to study the influence copper content and particle morphology on performance Cu/FeOx catalysts in gas-phase conversion CO2 with hydrogen. All four investigated a between 0 5 wt% were found highly efficient, reaching 36.8%, their selectivity towards C1 versus C2-C4, C2-C4=, C5+ products dependent catalyst composition, morphology, temperature. observed range is different ...

We surveyed more than 150 different materials as candidates for optically tunable (at 248 nm and 193 nm), attenuating embedded phase-shift masks. Multicomponent materials with four distinct microstructures: (1) composites, (2) cermets, (3) multilayers, and (4) copolymers, where one component was optically clear at the application wavelength and the other component more optically absorbing, prov...

The alpha-ketoglutarate-dependent hydroxylases and halogenases employ similar reaction mechanisms involving hydrogen-abstracting Fe(IV)-oxo (ferryl) intermediates. In the halogenases, the carboxylate residue from the His(2)(Asp/Glu)(1) "facial triad" of iron ligands found in the hydroxylases is replaced by alanine, and a halide ion (X(-)) coordinates at the vacated site. Halogenation is thought...