|Programmable Active Memories (PAM) are a novel form of universal recon gurable hardware co-processor. Based on Field-Programmable Gate Array (FPGA) technology, a PAM is a virtual machine, controlled by a standard microprocessor, which can be dynamically and inde nitely recongured into a large number of application-speci c circuits. PAMs o er a new mixture of hardware performance and software v...

BACKGROUND During development of recombinant monoclonal antibodies in Chinese hamster ovary (CHO) cells, C-terminal amidated species are observed. C-terminal amidation is catalysed by peptidylglycine α-amidating monooxygenase (PAM), an enzyme known to be expressed in CHO cells. The significant variations between clones during clone selection, and the relatively high content of amidated species ...

|Programmable Active Memories (PAM) are a novel form of universal recon gurable hardware co-processor. Based on Field-Programmable Gate Array (FPGA) technology, a PAM is a virtual machine, controlled by a standard microprocessor, which can be dynamically and inde nitely recongured into a large number of application-speci c circuits. PAMs o er a new mixture of hardware performance and software v...

We reported previously that protein associated with Myc (PAM) interacts with the C2 domain of type V adenylyl cyclase (ACV-C2) and that purified PAM is a potent inhibitor of Galphas-stimulated ACV activity (J Biol Chem 276:47583-47589, 2001). The present study was conducted to identify the region in PAM that inhibits ACV activity and to determine whether its binding with the ACV-C2 is necessary...

In this paper we present an experimental analysis of several modulation formats (pulse amplitude (PAM-2), quaternary pulse (PAM-4) and electrical duobinary (EDB)) for passive optical network (PON) applications at 25 Gbps bit rate in a C-band 10G-class directly modulated lasers (DML) avalanche photodiode (APD) intensity direct detection (IM-DD) system over single mode fiber (SMF) up to km, optim...

Cone snails, genus Conus, are predatory marine snails that use venom to capture their prey. This venom contains a diverse array of peptide toxins, known as conotoxins, which undergo a diverse set of posttranslational modifications. Amidating enzymes modify peptides and proteins containing a C-terminal glycine residue, resulting in loss of the glycine residue and amidation of the preceding resid...

Motivated by the need to develop instrumental techniques for characterizing organic aerosol aging, we report on the performance of the Toronto Photo-Oxidation Tube (TPOT) and Potential Aerosol Mass (PAM) flow tube reactors under a variety of experimental conditions. The PAM system was designed with lower surface-area-tovolume (SA/V) ratio to minimize wall effects; the TPOT reactor was designed ...

The proliferation of CRISPR/Cas9-based methods in Caenorhabditis elegans has enabled efficient genome editing and precise genomic tethering of Cas9 fusion proteins. Experimental designs using CRISPR/Cas9 are currently limited by the need for a protospacer adjacent motif (PAM) in the target with the sequence NGG. Here we report the characterization of two modified Cas9 proteins in C. elegans tha...

Short-term temperature effects on photosynthesis were investigated by measuring O2 production, PSII-fluorescence kinetics, and (14) C-incorporation rates in monocultures of the marine phytoplankton species Prorocentrum minimum (Pavill.) J. Schiller (Dinophyceae), Prymnesium parvum f. patelliferum (J. C. Green, D. J. Hibberd et Pienaar) A. Larsen (Coccolithophyceae), and Phaeodactylum tricornutu...