Green chemistry - also called sustainable chemistry - as the cost-effective and environmentally friendly techniques have been gaining more attention recent years. Here, we introduced a fast, non-toxic and sustainable method to synthesize Ag nanoparticles. Various parameters are involved in the bio-synthesis of Ag nanoparticles/multi-walled carbon nanotubes (Ag/MWf-CNT) composites, including...

Plackett-Burman design was used for selection of important media components such as carbon and nitrogen sources and minerals which affect poly(hydroxybutyrate) production and cell growth of Methylobacterium extorquens DSMZ 1340. Among the studied variables, nitrogen and phosphorus sources, MgSO4 and most of the trace elements were found to be significant variables for PHB product...

Dispersive liquid–liquid microextraction (DLLME) combined with inductively coupled plasma-atomic emission spectrometry (ICP-AES) was applied for the determination of lead in different environmental water samples. Ammonium pyrrolidine dithiocarbamate (APDC), chloroform and ethanol were used as chelating agent, extraction solvent and disperser solvent, respectively. The effective parameters, such...

Nisin is a bacteriocin approved in more than 50 countries as a safe natural food preservative. Response surface methodology (RSM) combined with artificial neural network-genetic algorithm (ANN-GA) was employed to optimize the fermentation medium for nisin production. Plackett-Burman design (PBD) was used for identifying the significant components in the fermentation medium. After that, the path...

Pectic-Oligosaccharides (POS) have a growing potential in food and feed industries. To satisfy the demand of worldwide markets from POS and avoid the shortcomings of currently applied methodologies encountered in their preparation, the present study highlights a novel robust approach for POS biosynthesis. In the current approach, Aspergillus sp.section Flavi strain EGY1 DSM 101520 was grown on ...

Bio-succinic acid production using microorganisms has been interesting as an environmentally friendly process. Palm oil mill effluent (POME) was considered a cheap substrate to lower the cost of production. It revealed that 2-fold diluted POME produced more succinic than undiluted and 5-fold POME. In addition, effects various neutralizing agents on utilized manage pH CO2 supply indicated utiliz...

background and objectives: nowadays corn flakes are the most common form of breakfast cereals however, their vitamins are declined during the baking process. in this research, production of fortified corn flakes by lactobacillus plantarum and l. reuteri, as well as incorporation of oat and malt fiber as prebiotics have been studied. materials and methods: plackett-burman statistical design was ...

A moderately halotolerant Streptomyces strain, designated JAJ13 was characterized and a culture medium was statistically optimized to improve its antibacterial activity. Based on the phenotypic and molecular characteristics, strain JAJ13 was identified as a moderately halotolerant Streptomyces sp. JAJ13. Novelty of the strain JAJ13 in production of antibacterial compound was assessed by sequenc...

While chiral 2,3-Butanediol (2,3-BD) is currently receiving remarkable attention because of its numerous industrial applications in the synthetic rubber, bioplastics, cosmetics, and flavor industries, 2,3-BD-mediated feedback inhibition of Paenibacillus polymyxa DSM 365 limits the accumulation of higher concentrations of 2,3-BD in the bioreactor during fermentation. The Box-Behnken design, Plac...

The screening of pollutant-degrading bacteria are limited due to most of bacteria in the natural environment cannot be cultivated. For the purpose of resuscitating and stimulating "viable but non-culturable" (VBNC) or uncultured bacteria, Micrococcus luteus proteins are more convenient and cost-effective than purified resuscitation-promoting factor (Rpf) protein. In this study, medium compositi...