Leptin, the 16-kDa product of the obese (ob) gene, primarily secreted from adipose tissue, has been implicated to play an important role in the regulation of food intake and energy expenditure. This study investigated protective effect of leptin on trichostatin A-induced apoptotic on in vitro maturation ratio of buffalo oocytes. Ovaries were collected from abattoir and were transported immediat...

The present study was designed to compare fertilization rates following oocyte exposure to spermatozoa from different buffalo bulls, using three sperm preparation methods i.e. percoll density gradient, swim-up (modified Ca free Tyrode’s medium and TALP medium) and sodium citrate washing prior to co-incubation with oocytes. Buffalo oocytes were aspirated from ovarian follicles within 1.5 to 2 ho...

The objective of the present study was to retrieve good and excellent quality of oocytes from the ovaries of buffalo having unknown reproductive history by using the aspiration technique. The retrieval as well as recovery percent was recorded. Total nine hundred and twenty ovaries were collected during the period of study, on an average 20 ovaries were collected per slaughter. Buffalo cumulus-o...

Buffalo ovaries were collected from a slaughter house and cumulus cells oocytes complexes (COCs) were recovered for preparation of 2-D, 4-D, 6-D and 8-D monolayer. Cumulus cells were stripped off by repeated pipetting into TCM-199 medium supplemented with 10% FCS. Approximately 2x106/ml cumulus cell were transferred in 100 μl drops of culture medium and incubated in humidifi ed atmosphere of 5%...

Background: The buffalo (Bubalus bubalis) is the animal with special production characteristics in producing milk and meat has a critical role in rural industry in Iran. The most problem of this specie is its low reproductive output. The aim of the present study was to evaluate the effect of bovine cumulus co-culture on maturation rate of Buffalo oocyte.Materials and Methods: Ovaries were colle...

The present study has been undertaken to assess the post thaw recovery of buffalo oocytes vitrified at different stages of in vitro maturation (IVM) by propylene glycol and trehalose supplemented with bovine serum albumin (BSA). Cumulus oocyte complexes (COCs) obtained from slaughterhouse ovaries were randomly divided into 6 different groups: control (nonvitrified oocytes were matured for 24 h ...

This study evaluated the effect of melatonin supplementation of in vitro maturation media on in vitro maturation (IVM) and in vitro fertilization (IVF) rate of buffalo oocytes. Cumulus oocytes complexes (COCs) were aspirated from follicles of 2-8 mm diameter. In experiment I, COCs were matured in IVM medium supplemented with 0 (control), 250, 500, and 1000 μM melatonin for 22-24 hours in CO2 in...

This paper aimed to study the dynamics of early embryonic development, in terms of redistribution of cytoskeleton (microtubules, actin microfilaments) and chromatin configurations during the first cell cycle in swamp buffalo embryos. Oocytes were matured and fertilized in vitro, and they were fixed at various time points after IVF. At 6 h after IVF, 44.4% matured oocytes were penetrated by sper...

The present study was undertaken to investigate the effects of ethylene glycol concentration and time of exposure to equilibration solution on the post-thaw morphological appearance and the in vitro maturation rate of buffalo oocytes. Vitrification solution-I (VS-I) consisted of 4.5M ethylene glycol (EG), 3.4M dimethyl sulphoxide, 5. 56mM glucose, 0.33mM sodium pyruvate and 0.4% w/v bovine seru...

The present study was conducted to investigate the effects of ovarian morphology on oocyte quantity and quality, as well as the effect of preincubated granulosa cells (PGCs) on in vitro maturation of buffalo oocytes and steroid hormone production. A total of 52 ovarian pairs were grouped into 3 types: Type I (with functional corpus luteum), Type II (with regressed corpus luteum), or Type III (w...