AIM To study the effect of preputial washing on bacterial load, preservability and semen quality in Murrah buffalo bulls. MATERIALS AND METHODS A total of 36 collections of three Murrah buffalo bulls maintained at Artificial Breeding Research Centre, ICAR-National Dairy Research Institute, Karnal, were collected at weekly intervals from each bull without preputial washing and latter ejaculate...

The present study was designed to see whether adding glutathione to tris-citric egg yolk extender improves the quality of buffalo bull spermatozoa stored at 5°C for five days. Semen collected from Nili-Ravi buffalo (Bubalus bubalis) bulls (n = 5) was diluted (37C; 10 x 10 motile spermatozoa per ml) with extender having glutathione (0.0, 0.5, 1.0, 2.0 and 3.0mM). The extended semen was cooled fr...

This study was designed to determine the effect of different osmotic pressures on spermatozoa characteristics for cryopreservation of buffalo bull semen using tris egg yolk extender (TEYE). Semen of Nili-Ravi buffalo bulls (n = 04) housed at Semen Production Unit, Qadirabad, Sahiwal, Pakistan, was collected at weekly interval for 10 weeks. Three solutions of tris-citric acid-fructose with osmot...

The objective of this experiment was to evaluate the effect of bull exposure on ovarian cyclicity in postpartum Murrah buffaloes. Twentyone buffaloes between the fi rst to fi fth lactation, which had calved during September and early February, were assigned at random to receive one of the following two treatments: (1) exposure to a vasectomized bull (bull exposed, BE) and (2) isolated from bull...

An Fn-II type group of protein present in seminal plasma of ungulates viz. heparin binding proteins (HBP) and PDC-I09 are considered as a new super family, that modulate the fertilizing ability of sperm (McCauley et al., 1999). Attempts to isolate these proteins from buffalo seminal plasma have been less successful. Although presence of proteins having af nity to heparin-Sepharose or gelatin-S...

Background: Optimal thawing rate is critical for quality and fertilizability of frozen -thawed spermatozoa. Kinetic parameters are the most important factors which are affected by thawing rate so, that have close relation between kinetic parameters and fertility of the spermatozoa. Tris - egg yolk have been widely used for cryopreservation of the buffalo and other species semen. According to ou...

Semen quality and its relationship to fertility are major concern in animal production. The aim of this study was to assess the relationship between frozen thawed sperm characteristics and fertility following in vivo fertility through Artificial Insemination(AI). Semen samples were collected from four buffalo bulls. Semen volume and sperm concentration appeared to be significantly different (P0...

Present study was conducted to the morphometric characteristics of slaughtered Murrah buffalo bull testis and its correlation with seminal attributes epididymal semen. Total 56 pair testicles were procured from Allana, slaughterhouse, Arrora, Mass agro food Pvt. Ltd. Unnao Uttar Pradesh, India; maintained at Deep Frozen Semen Laboratory, College Veterinary science, animal Husbadry, Ayodhya, Ind...