Using 'yolk sac chimaeras', we have previously demonstrated that stem cells, destined to colonize haemopoietic organs other than the yolk sac, arise in the embryo proper. We have now investigated the emergence and potentialities of these cells in vivo and in vitro. The in vivo approach consisted of interspecies grafting between quail and chick embryos. The cell progeny from the grafts was detec...

Plant mitochondrial and chloroplast genes that underwent horizontal transfer have been identified by parasite and grafting systems, respectively. Here, we directly observed 3 horizontal gene transfer (HGT) events in the 45 second axillary shoots of grafted cotton plants (Gossypium barbadense and Gossypium hirsutum) after extirpating the first axillary bud. The second axillary shoots showed phen...

The undifferentiated vertebrate limb bud is a selforganizing system. If transplanted to a favorable ectopic location, the limb bud is capable of developing into a morphologically normal limb (Harrison, 1918), and the anterior-posterior polarity of the transplanted limb is determined by the graft rather than the host environment. Great progress has recently been made in understanding the molecul...

The present investigation was carried out in the fruit nursery, Department of Fruit Science, College Horticulture and Forestry, Neri, Hamirpur, Himachal Pradesh w.e.f., June, 2019−July, 2020. In two factors viz., time wedge grafting (mid-June, mid-July, mid-August, mid-September, mid-February mid-March) use polycap (Graft union covered with or without polycap) were studied to standardize ideal ...

Reports on the stages when chick flank ectoderm can respond to ridge induction are contradictory. Different results have been obtained using presumptive wing or leg bud mesoderm as the inducing tissue with flank ectoderm as the responding tissue. In addition, although incomplete outgrowths have been obtained from recombinants with stage-19 flank ectoderm in a small percentage of cases, no compl...

To investigate the role of fibroblast growth factor-2 (basic fibroblast growth factor) in chick limb development, we constructed a replication-defective spleen necrosis virus to ectopically express fibroblast growth factor-2 in stage 20-22 chick limb bud. Because infecting cells in vivo proved to be inefficient, limb bud cells were dissociated, infected in vitro, and then grafted back into host...

Reports on the stages when chick flank ectoderm can respond to ridge induction are contradictory. Different results have been obtained using presumptive wing or leg bud mesoderm as the inducing tissue with flank ectoderm as the responding tissue. In addition, although incomplete outgrowths have been obtained from recombinants with stage-19 flank ectoderm in a small percentage of cases, no compl...

abstract among the propagation methods of mango, grafting is the most common method, but growers do not have enough knowledge about the suitable time and grafting methods of this fruit tree. this problem is the most important factor in production of grafted mango plants. this experiment was conducted in a randomized complete block design with factorial arrangement and 10 replications. factors i...

The susceptible species Cedrela odorata and Swietenia macrophylla to attack by Hypsipyla grandella (Zeller) larvae were grafted onto the resistant species Khaya senegalensis and Toona ciliata. Six-month-old grafted plants were then compared to their reciprocal grafts and to both intact (non-grafted) and autografted plants for damage due to H. grandella larvae and for their effects on larval per...