The objective of this study was to determine seroprevalence of small ruminant lentivirus (SRLV), Brucella abortus and Brucella melitensis infections among dairy goats in South Backa and Srem region in Vojvodina Province. The other aim was to determine the risk factors associated with SRLV infection in investigated herds. A total of 971 serum samples were collected from 8 comercial dairy goat he...

Brucella melitensis biotype 1 was isolated in pure culture from the lungs, liver, spleen, kidney, stomach contents, abomasum and brain of an aborted caprine (Boer goat) foetus in the district of Cullinan near Pretoria. The 18 does and 1 ram in the flock of Boer goates were examined serologically by means of the complement fixation (CF) test, using Brucella abortus antigen. Six weeks later they ...

A comparative study of the Rose Bengal test (RBT), Enzyme-Linked Immunosorbent Assay (ELISA) and complement fixation test (CFT) was performed to analyze sera of two Awassi flocks. Flock A was composed of 215 ewes, naturally infected with brucellosis. None of the animals were vaccinated. Flock B consisted of 336 non-infected non-vaccinated ewes. These served as negative controls. The two flocks ...

We developed an assay to detect antibodies spontaneously secreted in vitro by peripheral blood mononuclear cells (PBMC) against Brucella spp. High levels of anti-Brucella immunoglobulin G (IgG) and/or IgM and/or IgA antibodies were detected in the cell supernatant solution of PBMC cultures for 12 patients suffering from acute or focalized brucellosis and for 5 patients recently vaccinated again...

To fulfill the need for a simple and rapid diagnostic test for human brucellosis, we used the immunochromatographic lateral flow assay format to develop two assays, one for the detection of Brucella-specific immunoglobulin M (IgM) antibodies and one for the detection of Brucella-specific IgG antibodies. The diagnostic values of these tests were examined. The tests are shown to detect acute, per...

BACKGROUND Human brucellosis remains to be a neglected zoonotic disease among agro-pastoral communities where livestock rearing is one of the main economic activities. This study was conducted in different agro-pastoral communities in Sengerema district, Mwanza, Tanzania, to determine seroprevalence and predictors of anti-Brucella antibodies, information that may influence public awareness on t...

A cross-sectional study was conducted in order to detect antibodies for Brucella canis (B. canis) in dogs from urban Harare and five selected rural communities in Zimbabwe. Sera from randomly selected dogs were tested for antibodies to B. canis using an enzyme-linked immunosorbent assay. Overall, 17.6% of sera samples tested (57/324, 95% CI: 13.5-21.7) were positive for B. canis antibodies. For...

Brucellosis is a widespread and endemic disease in the Middle East Mediterranean regions, it has significant public health livestock industry concerns. It can affect wide range of mammalian hosts, including humans. This study aimed to determine seroprevalence thelatent infection brucellosisin calves; Rose Bengal indirect ELISA tests were conducted for this purpose. The serum samples collected f...

Human brucellosis is most commonly diagnosed by serology based on agglutination of fixed Brucella abortus as antigen. Nucleic acid amplification techniques have not proven capable of reproducibly and sensitively demonstrating the presence of Brucella DNA in clinical specimens. We sought to optimize a monoclonal antibody-based assay to detect Brucella melitensis lipopolysaccharide in blood by co...