BACKGROUND Brachyspira pilosicoli is an anaerobic spirochaete that can colonizes the large intestine of many host species. Infection is particularly problematic in pigs and adult poultry, causing colitis and diarrhea, but it is also known to result in clinical problems in human beings. Despite the economic importance of the spirochaete as an animal pathogen, and its potential as a zoonotic agen...

Two of four weak β-hemolytic isolates of intestinal spirochetes isolated from pigs in Japan possessed a unique base alignment of TTTTTT on the 16S ribosomal DNA of Brachyspira pilosicoli and were identified as B. pilosicoli. The other two isolates were not identified by this technique. The identified isolates were 4.2 to 11 μm in length and 0.2 to 0.3 μm in diameter, 4 periplasmic flagella at e...

BACKGROUND:Avian intestinal spirochetosis (AIS) is causedby spiral-shaped Gram-negative Brachyspira spp. in poultry. It isknown as a cause of diarrhea, low egg production, and increasedoccurrence of dirty eggs in layer hens. OBJECTIVES: In this study,the presence of some Brachyspira spp. was investigated in layinghens. METHODS: A total of 100 cloacal swab samples wereindividually collected from...

Avian intestinal spirochetosis (AIS) is a disease of birdscharacterized by a marked colonization of the cecum and/or rectumwith anaerobic intestinal bacteria of the genus Brachyspira. Thepurpose of the study was to determine the occurrence of Brachyspiraspp from avian sources in Iran and to characterize selected isolates bybiochemical and molecular methods. Out of 165 fecal samples obtained fro...

Brachyspira hyodysenteriae and Brachyspira pilosicoli are well-known intestinal pathogens in pigs. B. hyodysenteriae is the causative agent of swine dysentery, a disease with an important impact on pig production while B. pilosicoli is responsible of a milder diarrheal disease in these animals, porcine intestinal spirochetosis. Recent sequencing projects have provided information for the genome...

Previously-developed PCR protocols specific for the 16S rRNA gene of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli were adapted for the detection of these species in human faeces, following DNA extraction and purification using mini-prep columns. The limits of detection in seeded faeces for B. aalborgi and B. pilosicoli respectively were 2x10(2) and 7x10(3) cells p...

The in vitro antimicrobial susceptibility of the anaerobic intestinal spirochete Brachyspira pilosicoli was investigated by an agar dilution method. Human (n = 123) and porcine (n = 16) isolates were susceptible to metronidazole, ceftriaxone, meropenem, tetracycline, moxifloxacin, and chloramphenicol; erythromycin and ciprofloxacin were not active. Resistance to amoxicillin and clindamycin vari...

BACKGROUND The anaerobic spirochetes Brachyspira hyodysenteriae and Brachyspira pilosicoli cause diarrheal diseases in pigs. Their fastidious nature has hampered standardization of methods for antimicrobial susceptibility testing. For monitoring of antimicrobial susceptibility wild type cutoff values are needed to define where the wild type distribution of MICs ends and no approved cutoffs are ...

background:avian intestinal spirochetosis (ais) is causedby spiral-shaped gram-negative brachyspira spp. in poultry. it isknown as a cause of diarrhea, low egg production, and increasedoccurrence of dirty eggs in layer hens. objectives: in this study,the presence of some brachyspira spp. was investigated in layinghens. methods: a total of 100 cloacal swab samples wereindividually collected from...