نتایج جستجو برای: bovine prochymosin

تعداد نتایج: 73338  

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1983
J S Emtage S Angal M T Doel T J Harris B Jenkins G Lilley P A Lowe

A gene for calf prochymosin (prorennin) has been reconstructed from chemically synthesized oligodeoxyribonucleotides and cloned DNA copies of preprochymosin mRNA. This gene has been inserted into a bacterial expression plasmid containing the Escherichia coli tryptophan promoter and a bacterial ribosome binding site. Induction of transcription from the tryptophan promoter results in prochymosin ...

Journal: :Bioengineered 2014
Tessália Diniz Luerce Marcela Santiago Pacheco Azevedo Jean Guy LeBlanc Vasco Azevedo Anderson Miyoshi Daniela Santos Pontes

Bovine chymosin is an important milk-clotting agent used in the manufacturing of cheeses. Currently, the production of recombinant proteins by genetically modified organisms is widespread, leading to greatly reduced costs. Lactococcus (L.) lactis, the model lactic acid bacterium, was considered a good candidate for heterologous chymosin production for the following reasons: (1) it is considered...

2011
Hugo G Menzella

BACKGROUND Variations in codon usage between species are one of the major causes affecting recombinant protein expression levels, with a significant impact on the economy of industrial enzyme production processes. The use of codon-optimized genes may overcome this problem. However, designing a gene for optimal expression requires choosing from a vast number of possible DNA sequences and differe...

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