INTRODUCTION Homogeneity of sample achieved by adequate mixing of the blood sample is the key to the quality of analytical results. Immediate mixing of the sample upon blood collection insures that the anticoagulant in the specimen container has fully dissolved with the blood, thus maintaining not only the effective anticoagulant-to-blood ratio but also providing a specimen free from any blood ...

To simplify our procedure for blood sample processing for PCR, we introduced a simpler, shorter, and more cost-effective method for the separation of peripheral blood lymphocytes (PBL) and DNA extraction for amplification with Taq polymerase. By this method, blood samples are processed in two simple 15-min steps: (1) separation of PBLs from whole blood by red blood cell lysis with the Roche Spe...

conclusions in samples stored in plastic syringes, overestimation of pao2 levels should be noted if samples cooled before analysis. in samples stored in plastic syringes, it is not necessary to store samples in iced water when analysis delayed up to one hour. results pao2 of the samples stored at 0°c was increased significantly after 60 minutes (p = 0.007). the paco2 of the samples kept for 30 ...