OBJECTIVES Resistance to carbapenems in Aeromonas species is rare and mediated mostly by the chromosomal cphA gene. Our aims were to describe the molecular characteristics of the first cases of VIM-producing Aeromonas caviae isolated from human samples. METHODS Carbapenem-resistant Aeromonas (CRA) spp. were isolated from rectal surveillance cultures. Bacterial identification was done by dnaJ ...

Detecting colonization of patients with carbapenemase-producing bacteria can be difficult. This study compared the sensitivity and specificity of a PCR-based method (Xpert MDRO) for detecting blaKPC, blaNDM, and blaVIM carbapenem resistance genes using GeneXpert cartridges to the results of culture with and without a broth enrichment step on 328 rectal, perirectal, and stool samples. The cultur...

Sir, Acquired metallo-b-lactamases (MBLs) are the most common acquired carbapenemases in Pseudomonas aeruginosa. They confer a broad-spectrum b-lactam resistance profile, including resistance to the antipseudomonal penicillins, cephalosporins and carbapenems, that is not antagonized by the available b-lactamase inhibitors. Most MBL-producing strains exhibit a multidrug resistance profile, also ...

Việc gia tăng tỷ lệ Klebsiella pneumoniae kháng carbapenem đang trở thành mối lo của toàn thế giới.1 Các chủng do sinh men carbapenemase hoặc kết hợp với ESBL, AmpC. Trong nghiên cứu này, kiểu gen mã hóa được phát hiện bằng kỹ thuật PCR như blaKPC, blaNDM, blaOXA48, blaVIM, blaIMP trong số 187 carbapenemase. Kiểu phổ biến nhất là blaKPC (58,3%), blaNDM (21,4%) và blaOXA-48-like (18,7%). Nghiên ...

Background: Metallo-β-lactamases (MBLs) have been reported in gram negative bacilli and are becoming increasingly important clinically because the enzymes hydrolyse almost all β-lactams, including carbapenems. Thus, the present study was conducted to determine the prevalence of MBL types in imipenem-nonsusceptible Pseudomonas aeruginosa and Acinetobacter baumannii isolated from a tertiary teach...

Carbapenemase-producing Enterobacteriaceae and Pseudomonas spp. are increasingly reported in many countries all over the world. Due to the resistance of those bacteria to almost all antibiotics (e.g.beta-lactams, aminoglycosides, fluoroquinolones),treatment options are seriously limited. In the Czech Republic, the incidence of carbapenemase-producing Enterobacteriaceae seems to be low, restrict...

5 Abstract Aim: To analyse the occurrence of bla genes (blaIMP, blaVIM, blaGIM, blaSIM, blaSPM, blaPER, blaKPC, and blaOXA) responsible for carbapenemresistance, as well as the presence of cephalosporin-resistance genes (blaCTX-M, blaTEM, blaSHV and blaampC ) by polymerase chain reactions (PCRs). Methods: The study was performed on routine samples subjected to culture and antibiotics susceptibi...

BACKGROUND Multidrug-resistant Pseudomonas aeruginosa is a serious challenge for antimicrobial therapy of nosocomial infections, as it possesses several mechanisms of antimicrobial resistance. In Central Greece, a sudden increase of infections caused by carbapenem-resistant P. aeruginosa was observed during 2011, indicating the need for further analysis. METHODS Five-hundred and sixty-eight P...

INTRODUCTION The emergence and rapid spread of blaIMP and blaVIM metallo-beta-lactamase (MBL) producing Gram-negative bacteria causing nosocomial infections are of concern worldwide due to limited treatment options. METHODOLOGY A total of 179 nonreplicate, consecutive, carbapenem resistant Pseudomonas aeruginosa (61), Acinetobacter baumannii (116), Acinetobacter lwoffii (1) and Pseudomonas st...

Production of a metallo-beta-lactamase activity was detected in a carbapenem-resistant Pseudomonas aeruginosa clinical isolate (isolate VR-143/97) from an Italian inpatient at the Verona University Hospital (northern Italy). The metallo-beta-lactamase determinant was isolated from a genomic library of VR-143/97, constructed in an Escherichia coli plasmid vector, by screening for clones with red...