This study analyzed 42 Acinetobacter baumannii strains collected between 2009-2012 from different hospitals in Beyrouth and North Lebanon to better understand the epidemiology and carbapenem resistance mechanisms in our collection and to compare the robustness of pulsed field gel electrophoresis (PFGE), multilocus sequence typing (MLST), repetitive sequence-based PCR (rep-PCR) and blaOXA-51 seq...

Carbapenem resistance in Acinetobacter baumannii is typically mediated by the acquisition of class D -lactamases belonging to gene clusters of blaOXA-23-like, blaOXA-40-like, and blaOXA-58like (1). In the Enterobacteriaceae, carbapenem-hydrolyzing class D -lactamases are encoded by a dissimilar set of genes, the blaOXA-48-like genes (2). The presence of blaOXA-23 in Enterobacteriaceae is except...

Methods: The studied population included 59 MDRAB strains previously genotyped by PFGE and multilocus sequence typing. The search for the ADC (blaADC), CHO (blaOXA-51-like, blaOXA-23-like, blaOXA-40-like and blaOXA-58-like) and MBL (blaIMP, blaVIM, blaSIM-1, blaGIM-1, blaSPM-1 and blaNDM-1) genes, and for the ISs (ISAba1, ISAba2, ISAba3, ISAba4 and IS18) was done by PCR assays. The phenotypic p...

Production of the OXA-23 carbapenemase is the most common reason for the increasing carbapenem resistance in Acinetobacter spp. This study was conducted to reveal the genetic basis of blaOXA-23 dissemination in Acinetobacter spp. in China. A total of 63 carbapenem-resistant OXA-23-producing Acinetobacter sp. isolates, representing different backgrounds, were selected from 28 hospitals in 18 pro...

OBJECTIVES To evaluate the in vitro activity of doripenem in Acinetobacter baumannii (A. baumannii) clinical isolates that possess different OXA-type carbapenemases, and to evaluate the roles of these enzymes in the development of carbapenem resistance. METHODS This retrospective study was conducted with 25 A. baumannii isolates at Sakarya University Training and Research Hospital, Sakarya, T...

Colombian Acinetobacter baumannii strain ST920 was isolated from the sputum of a 68-year-old male patient. This isolate possessed blaOXA-72 and blaOXA-255-like genes. The assembled genome contained 4,104,098 pb and 38.79% G+C content. This is the first case reported of the coproduction (blaOXA-72 and blaOXA-255-like) of carbapenem-hydrolyzing class D β-lactamases (CHDLs) in Acinetobacter bauman...

Acinetobacter baumannii is one of the most important nosocomial pathogen worldwide. This study aimed to investigate virulence potential and genomic relatedness A. strains isolated from patients hospitalized in Military Medical Academy (MMA) by detecting OXA-type carbapenemases genes, biofilm-associated RAPD analysis. PCR was used detect blaoxa ISAba-1 genetic element, genes. The determined anal...

Việc gia tăng tỷ lệ Klebsiella pneumoniae kháng carbapenem đang trở thành mối lo của toàn thế giới.1 Các chủng do sinh men carbapenemase hoặc kết hợp với ESBL, AmpC. Trong nghiên cứu này, kiểu gen mã hóa được phát hiện bằng kỹ thuật PCR như blaKPC, blaNDM, blaOXA48, blaVIM, blaIMP trong số 187 carbapenemase. Kiểu phổ biến nhất là blaKPC (58,3%), blaNDM (21,4%) và blaOXA-48-like (18,7%). Nghiên ...