An internal standard method was previously developed to measure the concentration of a synthetic bitter peptide, beta-CN f193-209, by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The objective of this study was to evaluate the relationship between beta-CN f193-209 concentration in an aqueous extract of aged Cheddar cheese and bitterness intensity of ...

Subjects were asked to assess the bitterness of one 6-n-propyl-2-thiouracil (PROP) and two quinine HCl (QHCl) concentrations presented via filter papers of varying sizes. The number of taste papillae stimulated by these filter papers was counted in each individual. Whole mouth sensitivity to PROP was determined in a separate session. In support of other demonstrations of spatial summation, thes...

The bitterness of 11 commercial medicines was evaluated both by a multichannel taste sensor and in human gustatory sensation tests with 15 volunteers. For basic drugs with amino groups in the molecule, such as quinine, there was a comparatively strong relative response electric potential (mV) of channels 1 or 2, those containing negatively charged membranes and the bitterness determined by huma...

A new set of cocoa bars named Toscolata® were developed containing top-quality extra virgin olive oil, dried apples cultivars, and chestnut flour. The present work has been conducted to define the sensory profile of these products through tasting by trained experts and consumers to study the acceptability, preference, and quality perception. The four sensorial profiles of the bars differed in t...

• Drinking a bitter beverage increases happy participants' inclination to save money. • Drinking a bitter beverage decreases unhappy participants' inclination to save money. • Priming bitterness-related concepts increases happy participants' inclination to save money. • Priming bitterness-related concepts decreases unhappy participants' inclination to save money. • Drinking a bitter beverage de...

In addition to their recognized roles in intra- and inter-species signaling, bacterial quorum-sensing molecules have been implicated in inter-kingdom signaling. A new study in Pseudomonas aeruginosa suggests that mammalian bitter taste receptors may recognize bacterial quorum sensing molecules, and widens the scope of such inter-kingdom communication. Intestinal cells also harbor these receptor...

Genetic variation in the ability to taste the bitterness of 6-n-propylthiouracil (PROP) is a complex trait that has been used to predict food preferences and eating habits. PROP tasting is primarily controlled by polymorphisms in the TAS2R38 gene. However, a variety of factors are known to modify the phenotype. Principle among them is the salivary protein Ps-1 belonging to the basic proline-ric...

Bitterness perception in mammals is mostly directed at natural toxins that induce innate avoidance behaviours. Bitter taste is mediated by the G protein-coupled receptor TAS2R, which is located in taste cell membranes. One of the best-studied bitter taste receptors is TAS2R38, which recognizes phenylthiocarbamide (PTC). Here we investigate the sensitivities of TAS2R38 receptors to PTC in four s...

Investigating the influence of plastein reaction on reducing the bitterness and offending smell of protein hydrolysates from yellowfin tuna offal. Protein hydrolysates of yellowfin tuna offal was prepared firstly, then the factors including pH value, kinds of enzymes, temperature and substrate concentration on influencing the nitrogen recovery and bitterness were separately evaluated and the co...