The second series of flexible shape-modified nucleosides is introduced. The "fleximers" feature the purine ring systems split into their individual imidazole and pyrimidine components. This structural modification serves to introduce flexibility to the nucleoside while still retaining the elements essential for recognition. As a consequence, these structurally innovative nucleosides can more re...

Nanoelectronic devices offer substantial potential for interrogating biological systems, although nearly all work has focused on planar device designs. We have overcome this limitation through synthetic integration of a nanoscale field-effect transistor (nanoFET) device at the tip of an acute-angle kinked silicon nanowire, where nanoscale connections are made by the arms of the kinked nanostruc...

Fluorescent bioprobes are powerful analytical tools for studying biological activities in living systems. Fluorogens with aggregation-induced emission (AIE) characteristics have recently emerged as a new class of fluorescent bioprobes for biosensing and cell imaging. As discovered by Tang, AIE fluorogens are nearly nonemissive in molecular states with their intermolecular rotations being not re...

Driven by the high demand for sensitive and specific tools for optical sensing and imaging, bioprobes with various working mechanisms and advanced functionalities are flourishing at an incredible speed. Conventional fluorescent probes suffer from the notorious effect of aggregation-caused quenching that imposes limitation on their labelling efficiency or concentration to achieve desired sensiti...

Amperometric enzyme electrode probes have been constructed for the specific determination of L-lysine and used in batch and flow analysis. The enzyme lysine oxidase was immobilized on a preactivated polymer support which was placed on a platinum electrode. Additional blocking membranes conferred high stability, reproducibility and avoided electrochemical and enzyme interferences. Parameters inc...

With its output wavelength covering the infrared penetrating window of most biological tissues at 1,200-1,250 nm, the femtosecond Cr:forsterite laser shows high potential to serve as an excellent excitation source for the multiphoton fluorescence microscope. Its high output power, short optical pulse width, high stability, and low dispersion in fibers make it a perfect replacement for the curre...

Here we present a new, rapid, immunofiltration assay for simultaneous detection of HIV p24 and Hepatitis B Virus antigens. The assay platform is composed of a 13 mm nitrocellulose filter spotted with capturing bioprobes and inserted in a Swinnex syringe filter holder. Samples and reagents are flown through the nitrocellulose filter by manual pressure on the syringe. A colorimetric detection all...