The construction of minimum enzymes cocktails could facilitate the bioconversion of specified feedstocks into monosaccharides or XOS. In recent reported researches, the hemicelluloses and recombinant hemicellulases, including endo-ß-1,4-xylanases, ß-xylosidases, α-L-arabinofuranosidases, acetyl xylan esterase, feruloyl esterase and mannanase as well as their interaction mechanisms were investig...

This paper describes an optimization of planetary mill pretreatment and saccharification processes for improving biosugar production. Pitch pine (Pinus rigida) wood sawdust waste was used as biomass feedstock and the process parameters optimized in this study were the buffering media, the milling time, the enzyme quantity, and the incubation time. Glucose yields were improved when acetate buffe...

Accurate and robust monitoring of product and reactants in a complex bioconversion stream is essential for the development of effective process control strategies. To monitor a microbially-catalysed Baeyer-Villiger bioconversion of a cyclic ketone to an optically pure lactone, a near infrared (NIR) spectroscopic method has been developed. The reaction, catalysed by cyclohexanone monooxygenase f...

Soybean extract residue (scum), a waste of soybean oil production, was examined as a raw material for C17-ketosteroid production. As a model process, its bioconversion to 9α-hydroxyandrost4-ene-3,17-dione (9-OH-AD) by Mycobacterium sp VKM Ac-1817D was studied. The content of transformable sterols (sitosterol, stigmasterol and campesterol) in scum was estimated at ∼14%. The bioconversion of scum...

3-Hydroxypropionaldehyde (3-HPA) is of high industrial interest as a new platform intermediate from bioconversion of renewable materials because it can be converted into a number of large scale commodity chemicals. In this work we studied the production of 3-HPA from bioconversion of glycerol in a two-stage process. In the first stage active biomass is produced. The active biomass is then used ...

On the basis of earlier experimental studies of the aerobic bioconversion of organic wastes, the preferred values of operating parameters and the biochemical rate constants of oxidation to CO2 and H2O were identified. Energy and material balances were then constructed for a large, 3 m deep aerobic cell holding 1,440 tons of the 'wet' component of organic wastes (major organic constituent: [C6H1...

The optimization of process parameters for the bioconversion of activated sludge by Penicillium corylophilum was investigated using response surface methodology (RSM). The three parameters namely temperature of 33 degrees C, agitation of 150 r/min, and pH of 5 were chosen as center point from the previous study of fungal treatment. The experimental data on chemical oxygen demand (COD) removal (...

BACKGROUND Bark and bark-containing forest residues have the potential for utilization as raw material for lignocellulosic ethanol production due to their abundance and low cost. However, the different physical properties and chemical composition of bark compared to the conventionally used wood chips may influence the spruce-to-ethanol bioconversion process. This study assesses the impact of ba...